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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Anaeromyxobacter sp. (strain Fw109-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi138 – 1381MagnesiumUniRule annotation
Metal bindingi141 – 1411MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciASP404589:GHMT-997-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Anae109_0992
OrganismiAnaeromyxobacter sp. (strain Fw109-5)
Taxonomic identifieri404589 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
ProteomesiUP000006382 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Crossover junction endodeoxyribonuclease RuvCPRO_1000071029Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi404589.Anae109_0992.

Structurei

3D structure databases

ProteinModelPortaliA7H905.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.

Sequencei

Sequence statusi: Complete.

A7H905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALGIDPGS RRCGYGVVAR EGTRLVVVAS GVLAPRAERP VAERLARILD
60 70 80 90 100
GLAEVIRRAG PAECSIEQVF SGASVRSALV LGQARGVALA AAAQAGLPVF
110 120 130 140 150
EYAPSEVKLA FTGSGRATKD QMIRTAHMLL GATPGLSDEA DALALAVCHL
160 170 180 190 200
ARRAGRLAVP AVRNVAVSVP GVAGRARPLA LPPARERAAL AAARLRPSRR

DHRGLA
Length:206
Mass (Da):21,269
Last modified:September 11, 2007 - v1
Checksum:i4874872FDD3D1B9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000769 Genomic DNA. Translation: ABS25201.1.
RefSeqiWP_011985307.1. NC_009675.1.
YP_001378185.1. NC_009675.1.

Genome annotation databases

EnsemblBacteriaiABS25201; ABS25201; Anae109_0992.
KEGGiafw:Anae109_0992.
PATRICi20927123. VBIAnaSp113478_1045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000769 Genomic DNA. Translation: ABS25201.1.
RefSeqiWP_011985307.1. NC_009675.1.
YP_001378185.1. NC_009675.1.

3D structure databases

ProteinModelPortaliA7H905.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi404589.Anae109_0992.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS25201; ABS25201; Anae109_0992.
KEGGiafw:Anae109_0992.
PATRICi20927123. VBIAnaSp113478_1045.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciASP404589:GHMT-997-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fw109-5.

Entry informationi

Entry nameiRUVC_ANADF
AccessioniPrimary (citable) accession number: A7H905
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: May 27, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.