Reviewed, UniProtKB/Swiss-Prot A7H8A2 (DNLJ_ANADF)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	Anae109_0735

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity.
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	DNA ligase HAMAP MF_01588		PRO_0000313112
Regions
	Domain	621 – 699	79	BRCT
	Nucleotide binding	38 – 42	5	NAD By similarity
	Nucleotide binding	87 – 88	2	NAD By similarity
Sites
	Active site	123	1	N6-AMP-lysine intermediate By similarity
	Metal binding	429	1	Zinc By similarity
	Metal binding	432	1	Zinc By similarity
	Metal binding	447	1	Zinc By similarity
	Metal binding	452	1	Zinc By similarity
	Binding site	121	1	NAD By similarity
	Binding site	144	1	NAD By similarity
	Binding site	186	1	NAD By similarity
	Binding site	311	1	NAD By similarity
	Binding site	335	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7H8A2-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 39A335F0F1A831C0	FASTA	699	77,167
	10 20 30 40 50 60 MRADADRDSA ETRAAELRAR ILDADHAYYV LDRPVLADAE YDRLVRELAE IEAAHPELAT 70 80 90 100 110 120 SDSPTQRVSG APSERFERVV HREPMLSLGN IQSDGELDEF DARVHRLLGL AGDVQVGYVA 130 140 150 160 170 180 EPKLDGLAVE LVYEGGRLVQ GSTRGDGVNG EDVTANLRTV GRTRANRGVP ERLAGEVPPR 190 200 210 220 230 240 VEVRGEVLLF KEHFEAMNRQ ILRAGDEPFA NPRNAAAGSL RQLDWRVTAR RPLSFIAYEA 250 260 270 280 290 300 LVPGAEPGGA RAGPWAWRTH AEKLEALARW GFETNAENER CEGIAAVKAY RDRMAERRFA 310 320 330 340 350 360 LPYDTDGVVV KVDDLDWRRR LGAASKFPRW AVAFKYPPQE EATRIRRIWA SVGRTGILTP 370 380 390 400 410 420 VVDFEPVRLS GAMVSRATLH NEDEMRRKDV LEGDWILVRR AGEVIPEVVM PLKERRTGEE 430 440 450 460 470 480 RPFAFPAECP ICGAKVVREE GEKVYRCTGA ACPAQLVGRL THFAQRRAMD IDGLGDKLAL 490 500 510 520 530 540 GLVERGLVKD FADLYAVPFP AWQELFSRPR KEERDAKAEL PQKSAQNMVD ALERSKATTL 550 560 570 580 590 600 RRLLFALGIP QVGEATAATL ARHFGELARF MDASEDALLA VRDVGPETAR EIRAWTEEPQ 610 620 630 640 650 660 NRRVVERLLA AGVRPAPETV DAGGPFAGKT VVLTGGLAAL SRDDAKAEIE RRGGKVSGSV 670 680 690 SRKTDLVVAG AEAGSKLEKA QALGVRVVGE EEFLRMLEE

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS24948.1.
RefSeq	YP_001377932.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7H8A2.
Genome annotation databases
GeneID	5376279.
GenomeReviews	Gene locus Anae109_0735 in contig CP000769_GR.
KEGG	afw:Anae109_0735.
NMPDR	fig|404589.4.peg.655.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YKFPAQE.
Family and domain databases
HAMAP	MF_01588. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
ProDom	PD003944. DNAligase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 3 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_ANADF

Accession

Primary (citable) accession number: A7H8A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)