Reviewed, UniProtKB/Swiss-Prot A7H7J7 (SYP_ANADF)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Anae109_0478

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_1000069118

Sequences

	Sequence		Length	Mass (Da)
	A7H7J7-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 8899C540647D9ECD	FASTA	575	63,091
	10 20 30 40 50 60 MPVVRLSQAF VPTLKEAPAD AQVASHKLLV RAGFIRQLGA GIYDYLPLAK RTLAKIEAIV 70 80 90 100 110 120 REEMDAIGGQ EFYLPALHPA EIWKESGRWE VMGDNMFRLK DRKNGDYCLG MTHEEIFTAI 130 140 150 160 170 180 ARDELRSYRQ LPQVWYQIQT KFRDEPRPKS GLLRVRQFTM KDAYSFDVDR AGLDKSYEDQ 190 200 210 220 230 240 RRAYERIFTR CGLDFVAVQA HSGAMGGSES SEFMVRTDAG EDLVAACPRC RYAANTETAT 250 260 270 280 290 300 SRLASEQDGA GLPKPEKFAT PGVVTIEALE QPPYGVAARR QLKTLVYVAD EKLVVAVVRG 310 320 330 340 350 360 DQELNEAKLQ TATGAQVIRP AHPEEIPSLM GARAGSLGAV GFSRAKVFVD PSLADRKDMV 370 380 390 400 410 420 TGANEDGFHL RGVEVRRDVL TGPHATVAEL RTVRAGEGCP RCDGTLDVFK ALEVGHIFKL 430 440 450 460 470 480 GTKYSESMKA TVLDADGKAV PIVMGSYGIG VERIMAAAIE LHHDELGIRW PPAIAPFQAT 490 500 510 520 530 540 VLTLGPEPEL KKAADELVKA LSDAGLEVLH DDREERAGVK FKDADLVGIP LRISVGKKGL 550 560 570 AEGKVEWKLR GDKAVELVPL AEVARRAAEH VRAGR

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS24693.1.
RefSeq	YP_001377677.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7H7J7.
Genome annotation databases
GeneID	5377703.
GenomeReviews	Gene locus Anae109_0478 in contig CP000769_GR.
KEGG	afw:Anae109_0478.
NMPDR	fig|404589.4.peg.427.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ANADF

Accession

Primary (citable) accession number: A7H7J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)