A7H513 (LSPA_CAMJD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	JJD26997_1596

Organism

Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) [Complete proteome] [HAMAP]

Taxonomic identifier

360109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097242
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Transmembrane	122 – 142	21	Helical; Potential
Sites
	Active site	104	1	By similarity
	Active site	130	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7H513 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 0B7BE3F1BBC30CBB	FASTA	156	18,290
	10 20 30 40 50 60 MAKTFKFIFY FWGAFVLVFA LDQWVKSLTL AGLRWQSKYL DLTYALNTGV AFSMLSFLEH 70 80 90 100 110 120 NLKYLHLALI GVLFIYLFWQ KTLLKTHSIA FGMMLGAGVS NLLDRFIYGG VVDMFFWHKW 130 140 150 FNFAIFNVAD VMINISVALI LIQEIFKKRK KDDRMD

References

[1]

"Complete genome sequence of Campylobacter jejuni subsp doylei 269.97 isolated from human blood." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1458 / RM4099 / 269.97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000768 Genomic DNA. Translation: ABS43452.1.
RefSeq	YP_001398593.1. NC_009707.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7H513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5389512.
GenomeReviews	Gene locus JJD26997_1596 in contig CP000768_GR.
KEGG	cjd:JJD26997_1596.
PATRIC	20048864. VBICamJej122183_1576.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HBG724422.
OMA	GVAFSMF.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	CJEJ360109:JJD26997_1596-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_CAMJD

Accession

Primary (citable) accession number: A7H513

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program