A7H4U5 (MURD_CAMJD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	JJD26997_1512

Organism

Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) [Complete proteome] [HAMAP]

Taxonomic identifier

360109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000056879
Regions
	Nucleotide binding	97 – 103	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7H4U5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: C67C73CCDCEF09D4	FASTA	402	45,398
	10 20 30 40 50 60 MKISLFGYGK TTCSIAENLV DKFGPFDIYD DHFTETKKDT LGNLLLNPND FDDNLSNIEI 70 80 90 100 110 120 PSPGFPPNHK LIQEAKNLQS EYDFFYDVMP KSVWISGTNG KTTTTQMAMH LLSHIGAVMG 130 140 150 160 170 180 GNVGTPLAEL DPYAKLWILE TSSFTLHYTH KAKPEIYALL PISPDHLSWH GSFKDYIQDK 190 200 210 220 230 240 LSVLKRMNEC DVAILPKIHT NTPTKAHIIS YEDEKDLATK FSIDTEKISF KSPFLLDAIM 250 260 270 280 290 300 ALAIEKILLD TLSYKLLNNF VMEKNKLEEI KDSQNRLWVN DTKATNESAV MAALNRYKDK 310 320 330 340 350 360 KIHLIIGGDD KGVDLSNLFD FMKGFDIELY AIGISTEKML NYAKKANLKA YKCEILQRAV 370 380 390 400 SEISNHLKVN EVALLSPACA SLDQFSSYAQ RGEVFKECIK KL

References

[1]

"Complete genome sequence of Campylobacter jejuni subsp doylei 269.97 isolated from human blood." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1458 / RM4099 / 269.97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000768 Genomic DNA. Translation: ABS44641.1.
RefSeq	YP_001398525.1. NC_009707.1.
3D structure databases
ProteinModelPortal	A7H4U5.
ModBase	Search...
Protein-protein interaction databases
STRING	A7H4U5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5390405.
GenomeReviews	Gene locus JJD26997_1512 in contig CP000768_GR.
KEGG	cjd:JJD26997_1512.
PATRIC	20048698. VBICamJej122183_1498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG321031.
OMA	DQFSSYK.
ProtClustDB	PRK03815.
Enzyme and pathway databases
BioCyc	CJEJ360109:JJD26997_1512-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CAMJD

Accession

Primary (citable) accession number: A7H4U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program