Reviewed, UniProtKB/Swiss-Prot A7H439 (NADE_CAMJD)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	JJD26997_1200

Organism

Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) [Complete proteome] [HAMAP]

Taxonomic identifier

360109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077541
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
Sites
	Active site	31	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7H439-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 46DDE3D546C15D50	FASTA	246	27,631
	10 20 30 40 50 60 MDWQKITEKM CDFIQEKVKN SQSQGVVLGL SGGIDSALVA TLCKRALKEN VFAFLMPTQI 70 80 90 100 110 120 SNKTNLEDAL RLCVDLNLEY KIIEIQSILD AFIKQSENTT LVSLGNFAAR IRMSLLYDYS 130 140 150 160 170 180 AMKNSLVIGT SNKSELLLGY GTIYGDLAYA FNPIGSLYKS EIYALAKYLN LHENFIKKTP 190 200 210 220 230 240 SADLWENQTD EADLGFSYAK IDEGLKALET NDEKLLKILD PSLIAMLKNR MQKNAFKGKM PEILEI

References

[1]

"Complete genome sequence of Campylobacter jejuni subsp doylei 269.97 isolated from human blood." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000768 Genomic DNA. Translation: ABS43563.1.
RefSeq	YP_001398269.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7H439.
Genome annotation databases
GeneID	5389724.
GenomeReviews	Gene locus JJD26997_1200 in contig CP000768_GR.
KEGG	cjd:JJD26997_1200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CAINPIG.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CAMJD

Accession

Primary (citable) accession number: A7H439

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)