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Protein

Thiazole synthase

Gene

thiG

Organism
Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100Schiff-base intermediate with DXPUniRule annotation1
Binding sitei161DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:JJD26997_0736
OrganismiCampylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97)
Taxonomic identifieri360109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000002302 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001246091 – 258Thiazole synthaseAdd BLAST258

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7H2Z6.
SMRiA7H2Z6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 188DXP bindingUniRule annotation2
Regioni209 – 210DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR033983. Thiazole_synthase_ThiG.
IPR008867. ThiG.
PfamiView protein in Pfam
PF05690. ThiG. 1 hit.

Sequencei

Sequence statusi: Complete.

A7H2Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENLKNDKL KIGKYEFDSR FILGSGKYSL ELIKSSIEEA KAQIITLALR
60 70 80 90 100
RANTGEIANI LDYIPKNITL LPNTSGARNA DEALRIARLS RELGCGELIK
110 120 130 140 150
IEVISDSRYL LPDNYETIKA CELLAKEGFT PLPYMHADLY AARAMRDVGA
160 170 180 190 200
AAIMPLAAPI GSNKGLCAKE FIQILLNEID LPIIVDAGIG TPAQACEAMQ
210 220 230 240 250
MGVSAVMVNT AIAEAKDIAL MARAFSLAVN AGRAAFLAGL ASVSEAKASS

PLTGFLRD
Length:258
Mass (Da):27,675
Last modified:September 11, 2007 - v1
Checksum:i816C60BAB5B0DEF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000768 Genomic DNA. Translation: ABS43917.1.
RefSeqiWP_011989984.1. NC_009707.1.

Genome annotation databases

EnsemblBacteriaiABS43917; ABS43917; JJD26997_0736.
KEGGicjd:JJD26997_0736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000768 Genomic DNA. Translation: ABS43917.1.
RefSeqiWP_011989984.1. NC_009707.1.

3D structure databases

ProteinModelPortaliA7H2Z6.
SMRiA7H2Z6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS43917; ABS43917; JJD26997_0736.
KEGGicjd:JJD26997_0736.

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR033983. Thiazole_synthase_ThiG.
IPR008867. ThiG.
PfamiView protein in Pfam
PF05690. ThiG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_CAMJD
AccessioniPrimary (citable) accession number: A7H2Z6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 11, 2007
Last modified: June 7, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.