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A7H2R6 (SYL_CAMJD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:JJD26997_0632
OrganismCampylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) [Complete proteome] [HAMAP]
Taxonomic identifier360109 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009317

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif579 – 5835"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7H2R6 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: C537E7C634CEE377

FASTA80993,268
        10         20         30         40         50         60 
MAYEASLIEK KWQKIWDENE YFEPKDDLNL PKKYILSMFP YPSGRIHMGH VRNYTIGDAL 

        70         80         90        100        110        120 
ARYYRKIGFN VLHPIGFDSF GMPAENAAIK HKIHPKSWTY ENIAYMKKEL FSLGFSFSKK 

       130        140        150        160        170        180 
RMLATSDPLY TKFEQEIFIK MFEKGLIYTK EANVNWCEQD QTVLANEQVE DGKCWRCGHE 

       190        200        210        220        230        240 
VVQKKMPGYY VKITAYAEKL LKDLEGLRDK WPNQVLTMQE NWIGKSEGLA FSLNLDEKSK 

       250        260        270        280        290        300 
QKAKESSLEV FTTRADTIYG VSYVALAPEH KIVQNLLLQN LLNQDVLNKI KAIQNQSPRE 

       310        320        330        340        350        360 
RQSSEKEGYF LGIYAIHPLS GEKIPLWVAN FVLSDYGSGA VMAVPAHDER DFEFAKKYNL 

       370        380        390        400        410        420 
AIKQVIQTQE NLPYTQKLGK LIHSQEFDNL DCNEARLKII SQFEAKNIGK RVVNFKIRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMIKCQSC GIAPQKLENL PITLPEDVQI TGEGNPLDKH PTWKNCICPK 

       490        500        510        520        530        540 
CGKEAQKESD TLDTFFESSW YFARFASDEK TWQEKALDEK SVKYWMSVDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYARFFQK ALRDLGYLTQ NEPFDRLLTQ GMVLKDGAKM SKSKGNVVDP DEIIEKYGAD 

       610        620        630        640        650        660 
TARLFMLFAA PPAKELEWND DAVEGAYRFI CKLYDRAQNI KKGELVELKQ ENLNKEEKYA 

       670        680        690        700        710        720 
RLKVYEALKK SEEVYHQSFA FNTLIAACME ALNALALCKN EALEQEAFYI ILNILEPIIP 

       730        740        750        760        770        780 
HVCFELSEEL FKCKNFKKLE LKEEVFVKDT LNLAVSINGK KRAEFKISSS ASKEEILAFA 

       790        800 
KENTAKWLEG KSIVKEIYVE GKLVNLVIK 

« Hide

References

[1]"Complete genome sequence of Campylobacter jejuni subsp doylei 269.97 isolated from human blood."
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1458 / RM4099 / 269.97.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000768 Genomic DNA. Translation: ABS43284.1.
RefSeqYP_001397796.1. NC_009707.1.

3D structure databases

ProteinModelPortalA7H2R6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360109.JJD26997_0632.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS43284; ABS43284; JJD26997_0632.
GeneID5390226.
KEGGcjd:JJD26997_0632.
PATRIC20046911. VBICamJej122183_0629.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCJEJ360109:GJDG-611-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAMJD
AccessionPrimary (citable) accession number: A7H2R6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries