Reviewed, UniProtKB/Swiss-Prot A7H2H6 (METE_CAMJD)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	JJD26997_0528

Organism

Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) [Complete proteome] [HAMAP]

Taxonomic identifier

360109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017234
Sites
	Metal binding	641	1	Zinc By similarity
	Metal binding	643	1	Zinc By similarity
	Metal binding	726	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7H2H6-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: FFBD9BBD92337AD5	FASTA	754	86,688
	10 20 30 40 50 60 MKNSIISYPR IGANRELKFA IEKYFKAQSS KEELLETAKN LRARHWKDIQ NAGIDFIPSN 70 80 90 100 110 120 DFSLYDNVLD TAVLFNIVHT KYKNLNLDAL DEYFAQSRGY QGENGDVTAL AMKKWFNTNY 130 140 150 160 170 180 HYLVPECDDA SIIALTGDKI FKEYLEAKEL GIESKPVLIG IFTLFKLIAF KDKKTQKLAK 190 200 210 220 230 240 EKLLNAYIEL FDKLNELKVT WLELDEPYLV YDLSKEDIAL FEEFYQELLN HKKDLKILLQ 250 260 270 280 290 300 SYFGDLRDIY PKLLESKFDA LGLDFIEGKQ SLALVQQYGF AKDKILFAGL INGKNIYAND 310 320 330 340 350 360 YAKSLKLIKE LQKYTQNIIL NTSCSLLHVP YSTEFESKLD PSYLKLFAFA KEKLQELKDL 370 380 390 400 410 420 KEILNSSEEN PLFKANQELF KNIPERLDEK VKARLKALKK EDFTRTPSFK ERALIQKEFL 430 440 450 460 470 480 KLPLLPTTTI GSFPQSADVR SNRLAFKQKK ISAQNYTEFN QQKIKECIQI QEEIGLDVLV 490 500 510 520 530 540 HGEFERNDMV EYFGENLKGF LFTQNGWVQS YGTRCVKPPI IWGDVSRTKP ITLACSKFAQ 550 560 570 580 590 600 SLSQKIVKGM LTGPVTILNW SFPREDISLK ESTEQIALAI RDEVLDLENA GIKIIQIDEA 610 620 630 640 650 660 ALREKLPLRK SDWHSEYLDW AIPAFNLVHS GVKAKTQIHT HMCYSEFSDI LKEIDAMDAD 670 680 690 700 710 720 VISFEASRSN LSLLDTLKAV HFKTEVGPGV YDIHSPRVPS VEELSLTIEK ILNKLPKEQI 730 740 750 WINPDCGLKT RAYEEVIASL KNLVTATQKI RKQL

References

[1]

"Complete genome sequence of Campylobacter jejuni subsp doylei 269.97 isolated from human blood." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000768 Genomic DNA. Translation: ABS44267.1.
RefSeq	YP_001397706.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7H2H6.
Genome annotation databases
GeneID	5390187.
GenomeReviews	Gene locus JJD26997_0528 in contig CP000768_GR.
KEGG	cjd:JJD26997_0528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CAMJD

Accession

Primary (citable) accession number: A7H2H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)