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A7H0I4 (BIOB_CAMC5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Ccur92_16720
ORF Names:CCV52592_0574
OrganismCampylobacter curvus (strain 525.92) [Complete proteome] [HAMAP]
Taxonomic identifier360105 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Biotin synthase HAMAP-Rule MF_01694
PRO_0000381281

Sites

Metal binding171Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2211Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A7H0I4 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: CF45D3A383859D97

FASTA27930,630
        10         20         30         40         50         60 
MKTIMLCAIS SLSAGNCSED CHYCTQSAGT KADIKRYKLK SPEQVVDEAK KAYANHALGF 

        70         80         90        100        110        120 
CLVTSGARLD DQKTEQVACI ARAVGKEVPQ LMLIACNGMA SYDQLKELKN AGVFSYNHNL 

       130        140        150        160        170        180 
ETSRDFFPKI CTTHSWDERW QTNLDAKATG LQLCCGGIYG IGESKTDRAS FRTSLKELAP 

       190        200        210        220        230        240 
FSSPINFFIA NPALKIKTPR LSVDEALKIV DDTVLALPNA RIMIAGGREA VLGERQYEIF 

       250        260        270 
DHGVSAVVIG DYLTTKGEES CKDIAKFKQM GFEFASKCH 

« Hide

References

[1]"Genome sequence of Campylobacter curvus 525.92 isolated from human feces."
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 525.92.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000767 Genomic DNA. Translation: EAU01333.1.
RefSeqYP_001408976.1. NC_009715.1.

3D structure databases

ProteinModelPortalA7H0I4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360105.CCV52592_0574.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEAU01333; EAU01333; CCV52592_0574.
GeneID5406212.
KEGGccv:CCV52592_0574.
PATRIC20033955. VBICamCur47627_1762.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycCCUR360105:GJ9P-1834-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CAMC5
AccessionPrimary (citable) accession number: A7H0I4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways