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A7GZP5 (NAPA_CAMC5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Ccur92_13830
ORF Names:CCV52592_1928
OrganismCampylobacter curvus (strain 525.92) [Complete proteome] [HAMAP]
Taxonomic identifier360105 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length926 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 926896Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069711

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A7GZP5 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: A5B0043D6D56F8B0

FASTA926104,203
        10         20         30         40         50         60 
MNRRDFIKSA AASAACASAG IAIPANLSAA SEAEKGWRWD KAACRFCGTG CGIMVATKEG 

        70         80         90        100        110        120 
KIVAVKGDPE APVNRGLNCI KGYFNAKIMY GEDRITHPLL RVNEKGEFDK KGKFKQVSWK 

       130        140        150        160        170        180 
QAFDVMEAQF RKTYDELGPH GVGVLGSGQY TIPEGYTAAK MMKAGFRSNS IDPNARHCMA 

       190        200        210        220        230        240 
SAVVGFIQVF GIDEPSGCFD DIELTDTIVA WGANMAEMHP ILWARVSDRK LSNPDRVKVV 

       250        260        270        280        290        300 
NLSTYSTRTS NLADIEIIFA PSADLAIWNY IAREIVYNHP EMIDEEFVKK HCIFTTGPAD 

       310        320        330        340        350        360 
IGYGLRPDIK YKKYLSSELD TAATEKSKVL SEAEGVTLSY LGLKAGDTME NKNTATAGNH 

       370        380        390        400        410        420 
WHISFEEFKK ALAPYTLEFT AKVAKGDPNE DIEEFKGKLK ALADLYIEKN RKVVSFWTMG 

       430        440        450        460        470        480 
FNQHQRGTWV NEQAYMVHFL LGKQALPGSG AFSLTGQPSA CGTAREVGTF VHRLPADMVV 

       490        500        510        520        530        540 
NNPKHREISE KIWKLPAGTL NGIPGSHYVK MMRDLEDGKV KFIWVQVNNP WQNTANANHW 

       550        560        570        580        590        600 
IKAAREMDNF IVVSDPYPGI SAKVADLILP TAMIYEKWGA YGNAERRTQH WRQQVLPVGE 

       610        620        630        640        650        660 
AMPDIWQMLE FSKRFKLKDV WGEKKLNDKV TLPSVLDAAK AMGYSEEDTL FDVLFANEEA 

       670        680        690        700        710        720 
KSYPANDPIM ENFDNTEVFG DKRGVIGSDG KEFKGYGFFV HKYLWEEYRK FGLGHGHDLA 

       730        740        750        760        770        780 
DFDTYHRVRG LRWPVVDGKE TQWRFNTKFD PYAKKAAPND KFAFYGNKAA ALPSGDLKGV 

       790        800        810        820        830        840 
TDKEKTPLTN KAKIFFRPYM DPCEMPSSEY PFWLCTGRVL EHWHSGTMTM RVPELYRAVP 

       850        860        870        880        890        900 
EALCYMHEQD AAKLGVLQNE IVWIESRRGK VKARVDLKGR NKPPVGLVYV PWFDENVFIN 

       910        920 
KVTLDSTCPI SKETDYKKCA VKIYKA 

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References

[1]"Genome sequence of Campylobacter curvus 525.92 isolated from human feces."
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 525.92.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000767 Genomic DNA. Translation: EAT99733.1.
RefSeqYP_001408687.1. NC_009715.1.

3D structure databases

ProteinModelPortalA7GZP5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360105.CCV52592_1928.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEAT99733; EAT99733; CCV52592_1928.
GeneID5407339.
KEGGccv:CCV52592_1928.
PATRIC20033344. VBICamCur47627_1458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycCCUR360105:GJ9P-1525-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CAMC5
AccessionPrimary (citable) accession number: A7GZP5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families