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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter curvus (strain 525.92)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei81Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei149Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei174Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei178Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei419Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei423Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei529Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei577Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei604Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei892Substrate; via amide nitrogenUniRule annotation1
Binding sitei900Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei917Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCCUR360105:G1G9H-1446-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Ccur92_13830
ORF Names:CCV52592_1928
OrganismiCampylobacter curvus (strain 525.92)
Taxonomic identifieri360105 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000006380 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_100006971131 – 926Periplasmic nitrate reductaseUniRule annotationAdd BLAST896

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi360105.CCV52592_1928

Structurei

3D structure databases

ProteinModelPortaliA7GZP5
SMRiA7GZP5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 934Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 218Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni554 – 555Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni816 – 825Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7GZP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFIKSA AASAACASAG IAIPANLSAA SEAEKGWRWD KAACRFCGTG
60 70 80 90 100
CGIMVATKEG KIVAVKGDPE APVNRGLNCI KGYFNAKIMY GEDRITHPLL
110 120 130 140 150
RVNEKGEFDK KGKFKQVSWK QAFDVMEAQF RKTYDELGPH GVGVLGSGQY
160 170 180 190 200
TIPEGYTAAK MMKAGFRSNS IDPNARHCMA SAVVGFIQVF GIDEPSGCFD
210 220 230 240 250
DIELTDTIVA WGANMAEMHP ILWARVSDRK LSNPDRVKVV NLSTYSTRTS
260 270 280 290 300
NLADIEIIFA PSADLAIWNY IAREIVYNHP EMIDEEFVKK HCIFTTGPAD
310 320 330 340 350
IGYGLRPDIK YKKYLSSELD TAATEKSKVL SEAEGVTLSY LGLKAGDTME
360 370 380 390 400
NKNTATAGNH WHISFEEFKK ALAPYTLEFT AKVAKGDPNE DIEEFKGKLK
410 420 430 440 450
ALADLYIEKN RKVVSFWTMG FNQHQRGTWV NEQAYMVHFL LGKQALPGSG
460 470 480 490 500
AFSLTGQPSA CGTAREVGTF VHRLPADMVV NNPKHREISE KIWKLPAGTL
510 520 530 540 550
NGIPGSHYVK MMRDLEDGKV KFIWVQVNNP WQNTANANHW IKAAREMDNF
560 570 580 590 600
IVVSDPYPGI SAKVADLILP TAMIYEKWGA YGNAERRTQH WRQQVLPVGE
610 620 630 640 650
AMPDIWQMLE FSKRFKLKDV WGEKKLNDKV TLPSVLDAAK AMGYSEEDTL
660 670 680 690 700
FDVLFANEEA KSYPANDPIM ENFDNTEVFG DKRGVIGSDG KEFKGYGFFV
710 720 730 740 750
HKYLWEEYRK FGLGHGHDLA DFDTYHRVRG LRWPVVDGKE TQWRFNTKFD
760 770 780 790 800
PYAKKAAPND KFAFYGNKAA ALPSGDLKGV TDKEKTPLTN KAKIFFRPYM
810 820 830 840 850
DPCEMPSSEY PFWLCTGRVL EHWHSGTMTM RVPELYRAVP EALCYMHEQD
860 870 880 890 900
AAKLGVLQNE IVWIESRRGK VKARVDLKGR NKPPVGLVYV PWFDENVFIN
910 920
KVTLDSTCPI SKETDYKKCA VKIYKA
Length:926
Mass (Da):104,203
Last modified:September 11, 2007 - v1
Checksum:iA5B0043D6D56F8B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000767 Genomic DNA Translation: EAT99733.1
RefSeqiWP_011992551.1, NC_009715.2

Genome annotation databases

EnsemblBacteriaiEAT99733; EAT99733; CCV52592_1928
KEGGiccv:CCV52592_1928

Similar proteinsi

Entry informationi

Entry nameiNAPA_CAMC5
AccessioniPrimary (citable) accession number: A7GZP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health