Reviewed, UniProtKB/Swiss-Prot A7GYY6 (PUR9_CAMC5)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	Ccur92_11240
ORF Names:	CCV52592_1838

Organism

Campylobacter curvus (strain 525.92) [Complete proteome] [HAMAP]

Taxonomic identifier

360105 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000018868

Sequences

	Sequence		Length	Mass (Da)
	A7GYY6-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 0E25DBA5862106AF	FASTA	510	56,584
	10 20 30 40 50 60 MRALISVSDK EGVVEFAKGL EQLGWQIVST GGTYKILNEN GVKAVEVAQI TQSPEMFEGR 70 80 90 100 110 120 VKTLHPKIHG GILYKRDDAS HVAQAKEFGI EGIDLVCVNL YPFKETTIRT DDFDEIIENI 130 140 150 160 170 180 DIGGPAMVRS AAKNFKDVLI VTSVLDYDEI LQRLKEGTDD LEFRRNLMIK AYEHTASYDA 190 200 210 220 230 240 TIANYMNERF KGGFGDARFI FGNKVFDTRY GENPHQKGAL YEFEYFFTNN FRALKGEASF 250 260 270 280 290 300 NNMTDINGAV MLATSFENAP VVAIVKHSNP CGLAVKDSLL ESYVEALKCD PISAYGGVVA 310 320 330 340 350 360 INGTLDRALA RKINEIYVEV IIAANVDDDA LAVFENKKRI KIFTQDNKFL VRAGDKFDFK 370 380 390 400 410 420 HIDGGFVFQE RDVVSDDELK NMKQMSKKHA NENQLKDAQI AWKVAALTKS NCVVYVKDGA 430 440 450 460 470 480 MVAIGMGMTS RVDAARAAVA KAKELDLDLH GCVLASEAFF PFRDSIDIAS KVGVKCVIEP 490 500 510 GGSIRDDEVI EAANEHGMSL YFTGVRHFLH

References

[1]	"Genome sequence of Campylobacter curvus 525.92 isolated from human feces." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000767 Genomic DNA. Translation: EAU00271.1.
RefSeq	YP_001408428.1.
3D structure databases
SMR	A7GYY6. Positions 2-510.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GYY6.
Genome annotation databases
GeneID	5406548.
GenomeReviews	Gene locus Ccur92_11240 in contig CP000767_GR.
KEGG	ccv:CCV52592_1838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HBG498048.
OMA	VVKHVKS.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_CAMC5

Accession

Primary (citable) accession number: A7GYY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)