A7GXI1 (SYE1_CAMC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Ccur92_06190
ORF Names:	CCV52592_0438

Organism

Campylobacter curvus (strain 525.92) [Complete proteome] [HAMAP]

Taxonomic identifier

360105 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367634
Regions
	Motif	6 – 16	11	"HIGH" region HAMAP MF_00022_B
	Motif	235 – 239	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	238	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GXI1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 3CFA961EB943E1A2	FASTA	431	49,152
	10 20 30 40 50 60 MYRFAPSPTG DMHIGNLRAA IFNYICSLQD KSGFILRIED TDKERNIQGK EKDILEILSK 70 80 90 100 110 120 FGIKPQQIYI QSENLKFHRQ LASKLLIDKK AFACFCTEEE LEAKKQKAKE EGVAYRYDGT 130 140 150 160 170 180 CERLSDAEVL ACDKPFVIRM KKPERTMSFT DAIKGELSFE PDAVDSFVIM RTDKTPTYNF 190 200 210 220 230 240 ACAVDDMLEG VTFVIRGEDH VSNTPKQDLI REGLGYTGKM NYAHLPILLN IEGKKMSKRE 250 260 270 280 290 300 NESSVKWLFE QGFLPEAIAN YLILLGNKTP SEIFTIDEAV KWFDITKISR SPARFDVKKL 310 320 330 340 350 360 EQINREHIKL ASDDRIAEVF GMDKNLANLV RFYTQESSLV PEIKEKVNKI FAPKVAPEEY 370 380 390 400 410 420 KSEFETIKNA AKNLGEFENF DDFKKALMTA TGLKGKNFFM PLRALLTGDL HGPELSELYP 430 LIKGDLARII A

References

[1]

"Genome sequence of Campylobacter curvus 525.92 isolated from human feces." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 525.92.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000767 Genomic DNA. Translation: EAU00724.1.
RefSeq	YP_001407923.1. NC_009715.1.
3D structure databases
ProteinModelPortal	A7GXI1.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GXI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5406794.
GenomeReviews	Gene locus Ccur92_06190 in contig CP000767_GR.
KEGG	ccv:CCV52592_0438.
PATRIC	20031690. VBICamCur47627_0662.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	VAYRYDG.
ProtClustDB	PRK12410.
Enzyme and pathway databases
BioCyc	CCUR360105:CCV52592_0438-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_CAMC5

Accession

Primary (citable) accession number: A7GXI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program