A7GXB5 (ISPH_CAMC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Ccur92_05530
ORF Names:	CCV52592_0515

Organism

Campylobacter curvus (strain 525.92) [Complete proteome] [HAMAP]

Taxonomic identifier

360105 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021094

Sequences

	Sequence		Length	Mass (Da)
	A7GXB5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 70565E729802DBB4	FASTA	274	30,618
	10 20 30 40 50 60 MKIELASSYG FCFGVKRAIK IAENAGDAAT IGPLIHNNEE INRLATNFNV KTLHGINELK 70 80 90 100 110 120 DEKKAIIRTH GITKSDLAEL KKTDIKVIDA TCPFVTKPQQ ICEDMSNAGY DVVIFGDENH 130 140 150 160 170 180 PEVKGVKSYA SGKVYVVLDE SELEGVKFRQ KVALVSQTTR KVEKFMQIAN YLMLRVKEVR 190 200 210 220 230 240 VFNTICNATF ENQEAVKNLA KRADVMIVIG GKNSSNTKQL YLISKNFCED SYLIESEHEV 250 260 270 EKSWFEGKNL CGISAGASTP DWIIQKVVDA IEKF

References

[1]

"Genome sequence of Campylobacter curvus 525.92 isolated from human feces." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 525.92.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000767 Genomic DNA. Translation: EAU00772.1.
RefSeq	YP_001407857.1. NC_009715.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7GXB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5405985.
GenomeReviews	Gene locus Ccur92_05530 in contig CP000767_GR.
KEGG	ccv:CCV52592_0515.
PATRIC	20031538. VBICamCur47627_0587.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	WGAPVYV.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	CCUR360105:CCV52592_0515-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_CAMC5

Accession

Primary (citable) accession number: A7GXB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program