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A7GX26

- SYI_CAMC5

UniProt

A7GX26 - SYI_CAMC5

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter curvus (strain 525.92)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
Binding sitei614 – 6141ATPUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi896 – 8961ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCUR360105:GJ9P-505-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ccur92_04640
ORF Names:CCV52592_0977
OrganismiCampylobacter curvus (strain 525.92)
Taxonomic identifieri360105 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000006380: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000022053Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi360105.CCV52592_0977.

Structurei

3D structure databases

ProteinModelPortaliA7GX26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7GX26-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTLLLP ATDFPMRGDL PKNEPIRLKS WYEERKIYEK MKSKRAGAAK
60 70 80 90 100
NFAIHDGPPY ANGHLHIGHA LNKILKDIIT KTHYFFGENV RYVPGWDCHG
110 120 130 140 150
LPIEQQVEVK LGDKKKELSK TQIRELCRAH AREFIDIQRE EFKALGIIGD
160 170 180 190 200
FENPYMTMKF EFEADIYRSL CEIAKKGLLI ERSKPVYWSW AAKSALAEAE
210 220 230 240 250
VEYEDKEDYS IYVAFELDSD ALAKLGVKLA KAVIWTTTPW TLPANQAISL
260 270 280 290 300
KPDEIYVLTS ENLIFAKPLL ESLVNLGLTK GEILKEFVSN ELENTHAINP
310 320 330 340 350
LNDRKSRFLL GDHVLMDGGT GLVHTAPGHG EDDYYICLRY GFKEILMPVD
360 370 380 390 400
DGGLYDETLK AHSLLRADVV DSFVGMHIFK ANEKIIELLG ENLLHVSKFT
410 420 430 440 450
HSYPFCWRTH KPVIYRATKQ WFIAMDEPKL GGKTLREVAR GELENVKFYP
460 470 480 490 500
SVGIKRIGSM IENRPDWCIS RQRDWGVPIA FFRRKDTKEP IFEPKILEHI
510 520 530 540 550
AKIFEQKGAD AWWDMSVEEL LAPDSGFEAK NLEKVMDILD VWFDSGSTWH
560 570 580 590 600
AVLNSKNYDA GSYPADMYLE GSDQHRGWFQ SSLLVSTAIN SHAPYKNILT
610 620 630 640 650
HGFTVDENGQ KMSKSKGNVV APQDVAKSYG VEILRLWVGL SDYSSDLKIS
660 670 680 690 700
ENILKQVSEQ YRKIRNTIRF LLANVNDLET IGTDFGFLDQ WILGRAKRVF
710 720 730 740 750
DEASKCFRAY DFSKGFNLLL NFLSADLSGI YLDICKDRLY CDAKDSPRRR
760 770 780 790 800
SAQSAMAIIT KSLLPLIAPT LTYTVDEVMD YAPAIIKGDA KDAFDLVYEP
810 820 830 840 850
INFDFDVEDE LLFASREKFF ELIDALKKDK KIKSTLELVL ETTSSKILDY
860 870 880 890 900
DSVERADIYM VSDVHRYSGN EGLGEFEIDG EKFKIVLSDA SKCPRCWKFN
910
AIIDGSTCER CSEVLNSVC
Length:919
Mass (Da):104,842
Last modified:September 11, 2007 - v1
Checksum:iC211CC0ADD97716E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000767 Genomic DNA. Translation: EAT99831.1.
RefSeqiYP_001407768.1. NC_009715.1.

Genome annotation databases

EnsemblBacteriaiEAT99831; EAT99831; CCV52592_0977.
GeneIDi5406413.
KEGGiccv:CCV52592_0977.
PATRICi20031348. VBICamCur47627_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000767 Genomic DNA. Translation: EAT99831.1 .
RefSeqi YP_001407768.1. NC_009715.1.

3D structure databases

ProteinModelPortali A7GX26.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360105.CCV52592_0977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EAT99831 ; EAT99831 ; CCV52592_0977 .
GeneIDi 5406413.
KEGGi ccv:CCV52592_0977.
PATRICi 20031348. VBICamCur47627_0493.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CCUR360105:GJ9P-505-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Campylobacter curvus 525.92 isolated from human feces."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E.
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 525.92.

Entry informationi

Entry nameiSYI_CAMC5
AccessioniPrimary (citable) accession number: A7GX26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3