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A7GX26

- SYI_CAMC5

UniProt

A7GX26 - SYI_CAMC5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter curvus (strain 525.92)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
    Binding sitei614 – 6141ATPUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation
    Metal bindingi896 – 8961ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCCUR360105:GJ9P-505-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Ccur92_04640
    ORF Names:CCV52592_0977
    OrganismiCampylobacter curvus (strain 525.92)
    Taxonomic identifieri360105 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000006380: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000022053Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360105.CCV52592_0977.

    Structurei

    3D structure databases

    ProteinModelPortaliA7GX26.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi611 – 6155"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7GX26-1 [UniParc]FASTAAdd to Basket

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    MDYKDTLLLP ATDFPMRGDL PKNEPIRLKS WYEERKIYEK MKSKRAGAAK    50
    NFAIHDGPPY ANGHLHIGHA LNKILKDIIT KTHYFFGENV RYVPGWDCHG 100
    LPIEQQVEVK LGDKKKELSK TQIRELCRAH AREFIDIQRE EFKALGIIGD 150
    FENPYMTMKF EFEADIYRSL CEIAKKGLLI ERSKPVYWSW AAKSALAEAE 200
    VEYEDKEDYS IYVAFELDSD ALAKLGVKLA KAVIWTTTPW TLPANQAISL 250
    KPDEIYVLTS ENLIFAKPLL ESLVNLGLTK GEILKEFVSN ELENTHAINP 300
    LNDRKSRFLL GDHVLMDGGT GLVHTAPGHG EDDYYICLRY GFKEILMPVD 350
    DGGLYDETLK AHSLLRADVV DSFVGMHIFK ANEKIIELLG ENLLHVSKFT 400
    HSYPFCWRTH KPVIYRATKQ WFIAMDEPKL GGKTLREVAR GELENVKFYP 450
    SVGIKRIGSM IENRPDWCIS RQRDWGVPIA FFRRKDTKEP IFEPKILEHI 500
    AKIFEQKGAD AWWDMSVEEL LAPDSGFEAK NLEKVMDILD VWFDSGSTWH 550
    AVLNSKNYDA GSYPADMYLE GSDQHRGWFQ SSLLVSTAIN SHAPYKNILT 600
    HGFTVDENGQ KMSKSKGNVV APQDVAKSYG VEILRLWVGL SDYSSDLKIS 650
    ENILKQVSEQ YRKIRNTIRF LLANVNDLET IGTDFGFLDQ WILGRAKRVF 700
    DEASKCFRAY DFSKGFNLLL NFLSADLSGI YLDICKDRLY CDAKDSPRRR 750
    SAQSAMAIIT KSLLPLIAPT LTYTVDEVMD YAPAIIKGDA KDAFDLVYEP 800
    INFDFDVEDE LLFASREKFF ELIDALKKDK KIKSTLELVL ETTSSKILDY 850
    DSVERADIYM VSDVHRYSGN EGLGEFEIDG EKFKIVLSDA SKCPRCWKFN 900
    AIIDGSTCER CSEVLNSVC 919
    Length:919
    Mass (Da):104,842
    Last modified:September 11, 2007 - v1
    Checksum:iC211CC0ADD97716E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000767 Genomic DNA. Translation: EAT99831.1.
    RefSeqiYP_001407768.1. NC_009715.1.

    Genome annotation databases

    EnsemblBacteriaiEAT99831; EAT99831; CCV52592_0977.
    GeneIDi5406413.
    KEGGiccv:CCV52592_0977.
    PATRICi20031348. VBICamCur47627_0493.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000767 Genomic DNA. Translation: EAT99831.1 .
    RefSeqi YP_001407768.1. NC_009715.1.

    3D structure databases

    ProteinModelPortali A7GX26.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360105.CCV52592_0977.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EAT99831 ; EAT99831 ; CCV52592_0977 .
    GeneIDi 5406413.
    KEGGi ccv:CCV52592_0977.
    PATRICi 20031348. VBICamCur47627_0493.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CCUR360105:GJ9P-505-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Campylobacter curvus 525.92 isolated from human feces."
      Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E.
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 525.92.

    Entry informationi

    Entry nameiSYI_CAMC5
    AccessioniPrimary (citable) accession number: A7GX26
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3