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A7GX26

- SYI_CAMC5

UniProt

A7GX26 - SYI_CAMC5

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Ccur92_04640, CCV52592_0977
Organism
Campylobacter curvus (strain 525.92)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylate By similarity
Binding sitei614 – 6141ATP By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi896 – 8961Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCUR360105:GJ9P-505-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Ccur92_04640
ORF Names:CCV52592_0977
OrganismiCampylobacter curvus (strain 525.92)
Taxonomic identifieri360105 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000006380: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligaseUniRule annotationPRO_1000022053Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi360105.CCV52592_0977.

Structurei

3D structure databases

ProteinModelPortaliA7GX26.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotationAdd
BLAST
Motifi611 – 6155"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7GX26-1 [UniParc]FASTAAdd to Basket

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MDYKDTLLLP ATDFPMRGDL PKNEPIRLKS WYEERKIYEK MKSKRAGAAK    50
NFAIHDGPPY ANGHLHIGHA LNKILKDIIT KTHYFFGENV RYVPGWDCHG 100
LPIEQQVEVK LGDKKKELSK TQIRELCRAH AREFIDIQRE EFKALGIIGD 150
FENPYMTMKF EFEADIYRSL CEIAKKGLLI ERSKPVYWSW AAKSALAEAE 200
VEYEDKEDYS IYVAFELDSD ALAKLGVKLA KAVIWTTTPW TLPANQAISL 250
KPDEIYVLTS ENLIFAKPLL ESLVNLGLTK GEILKEFVSN ELENTHAINP 300
LNDRKSRFLL GDHVLMDGGT GLVHTAPGHG EDDYYICLRY GFKEILMPVD 350
DGGLYDETLK AHSLLRADVV DSFVGMHIFK ANEKIIELLG ENLLHVSKFT 400
HSYPFCWRTH KPVIYRATKQ WFIAMDEPKL GGKTLREVAR GELENVKFYP 450
SVGIKRIGSM IENRPDWCIS RQRDWGVPIA FFRRKDTKEP IFEPKILEHI 500
AKIFEQKGAD AWWDMSVEEL LAPDSGFEAK NLEKVMDILD VWFDSGSTWH 550
AVLNSKNYDA GSYPADMYLE GSDQHRGWFQ SSLLVSTAIN SHAPYKNILT 600
HGFTVDENGQ KMSKSKGNVV APQDVAKSYG VEILRLWVGL SDYSSDLKIS 650
ENILKQVSEQ YRKIRNTIRF LLANVNDLET IGTDFGFLDQ WILGRAKRVF 700
DEASKCFRAY DFSKGFNLLL NFLSADLSGI YLDICKDRLY CDAKDSPRRR 750
SAQSAMAIIT KSLLPLIAPT LTYTVDEVMD YAPAIIKGDA KDAFDLVYEP 800
INFDFDVEDE LLFASREKFF ELIDALKKDK KIKSTLELVL ETTSSKILDY 850
DSVERADIYM VSDVHRYSGN EGLGEFEIDG EKFKIVLSDA SKCPRCWKFN 900
AIIDGSTCER CSEVLNSVC 919
Length:919
Mass (Da):104,842
Last modified:September 11, 2007 - v1
Checksum:iC211CC0ADD97716E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000767 Genomic DNA. Translation: EAT99831.1.
RefSeqiYP_001407768.1. NC_009715.1.

Genome annotation databases

EnsemblBacteriaiEAT99831; EAT99831; CCV52592_0977.
GeneIDi5406413.
KEGGiccv:CCV52592_0977.
PATRICi20031348. VBICamCur47627_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000767 Genomic DNA. Translation: EAT99831.1 .
RefSeqi YP_001407768.1. NC_009715.1.

3D structure databases

ProteinModelPortali A7GX26.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360105.CCV52592_0977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai EAT99831 ; EAT99831 ; CCV52592_0977 .
GeneIDi 5406413.
KEGGi ccv:CCV52592_0977.
PATRICi 20031348. VBICamCur47627_0493.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CCUR360105:GJ9P-505-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Campylobacter curvus 525.92 isolated from human feces."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Lastovica A.J., Nelson K.E.
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 525.92.

Entry informationi

Entry nameiSYI_CAMC5
AccessioniPrimary (citable) accession number: A7GX26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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