A7GUC8 (AMPA_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Bcer98_3532

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000077273
Sites
	Active site	271	1	Potential
	Active site	345	1	Potential
	Metal binding	259	1	Manganese 2 By similarity
	Metal binding	264	1	Manganese 1 By similarity
	Metal binding	264	1	Manganese 2 By similarity
	Metal binding	282	1	Manganese 2 By similarity
	Metal binding	341	1	Manganese 1 By similarity
	Metal binding	343	1	Manganese 1 By similarity
	Metal binding	343	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GUC8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: F876B95C31D750DC	FASTA	493	53,721
	10 20 30 40 50 60 MFQVQTELAN HGAVIVALFE EETSRFVQEL DKAFEGQLQG LLDEKELSTK KKSISKVHSL 70 80 90 100 110 120 GKTNVKRYYF VGLGKKEAYT TETLRASLSK TFKTLQAEKI QDAAILLDSF VTEKLDAIDV 130 140 150 160 170 180 AHIAAEVYCL GTYRLQTYKT DKKEHVELEK LVVITAEDAK EIEAALTVGY VHGRATNSAR 190 200 210 220 230 240 TLVNMPPNML TATKLAEYAV ELAEKYDMDY KVLEKEEMEE LGMGALLAVN QGSTEPPKMI 250 260 270 280 290 300 ALIYKGKEEW KDVIGLIGKG ITYDTGGYSL KPRDGMVGMK GDMGGAAAVL GAMEIIGELR 310 320 330 340 350 360 PEQNVIAIIP STDNVVSGTA FKPDDVITSM SGKTIEVLNT DAEGRLALAD GITYAKKLGA 370 380 390 400 410 420 NYLVDVATLT GGVIVALGNH TTGAMTNNET LFEQVLEASM ETDERIWQLP IFERDKERVR 430 440 450 460 470 480 NSKFADLNNS PGRDGHAVMA GTFLGEFAED TPWVHLDIAG TSDTTSTHDL GPAGATGVMV 490 RTLATLVERF GEE

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS23736.1.
RefSeq	YP_001376731.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GUC8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GUC8.
Protein family/group databases
MEROPS	M17.010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000036497; EBBACP00000035548; EBBACG00000036488.
GeneID	5343661.
GenomeReviews	Gene locus Bcer98_3532 in contig CP000764_GR.
KEGG	bcy:Bcer98_3532.
PATRIC	18935768. VBIBacCyt128034_3700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
GeneTree	EBGT00050000002613.
HOGENOM	HBG742580.
OMA	CTEEAQL.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_3532-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BACCN

Accession

Primary (citable) accession number: A7GUC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program