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A7GUA1 (GLGB_BACCN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Bcer98_3504
OrganismBacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]
Taxonomic identifier315749 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length645 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6456451,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000083065

Sites

Active site3091Nucleophile By similarity
Active site3521Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A7GUA1 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: E77F40A241FE1BD2

FASTA64576,146
        10         20         30         40         50         60 
MRAIHCLEER LEQFHTEKCY ESYQIFGAHL AVENGVHGVR FTVWAPRAKN LSVVGDFNEW 

        70         80         90        100        110        120 
NEKQHRMQKV TEAGIWSLFI PQIEEKEIYK YAIETINGEV ILKADPYATY AEVRPNTASV 

       130        140        150        160        170        180 
ILDIEGYEWN DKNWFRKKKK KSVYKEAMAI YELHFGSWKK KEDGSLYSYR EMAEELIPYM 

       190        200        210        220        230        240 
ANHHFTHIEI MPLVEHPYDR SWGYQGTGYY AVTSRFGTPH DFMYFVDECH KYGIGVILDW 

       250        260        270        280        290        300 
VPGHFCKDAH GLYLFDGTPT YEYRDLDVQE NRVWGTANFD LGKREVRNFL ISNALFWMKY 

       310        320        330        340        350        360 
YHIDGFRVDA VANMLYWEKE GKAQSNEYAV SFLRELNEAV FAEDGEFLMT AEDSTAWPLV 

       370        380        390        400        410        420 
TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHEK MTFSLLYAYS ENFILPLSHD 

       430        440        450        460        470        480 
EVVHGKKSLL NKMPGNYWEK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 

       490        500        510        520        530        540 
NLHEFEMHRH MHDYFKELIA LYKRSKPLWQ LDYSHEGFQW IDADNKEQSI FSFIRKGDRE 

       550        560        570        580        590        600 
DDVLIVICNF TSIVYEKYKV GVPEFQYYNE ILNSDAIAYG GSGRINKKRL KSISQPYHNQ 

       610        620        630        640 
TAHVEITIPP FGVSILRPVK MRKGSKKQDG KKAELRSNAT SRRKR 

« Hide

References

[1]"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity."
Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A.
Chem. Biol. Interact. 171:236-249(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NVH 391-98.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000764 Genomic DNA. Translation: ABS23709.1.
RefSeqYP_001376704.1. NC_009674.1.

3D structure databases

ProteinModelPortalA7GUA1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315749.Bcer98_3504.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS23709; ABS23709; Bcer98_3504.
GeneID5347533.
KEGGbcy:Bcer98_3504.
PATRIC18935660. VBIBacCyt128034_3671.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAPIGVAYK.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBCYT315749:GH2A-3613-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BACCN
AccessionPrimary (citable) accession number: A7GUA1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries