Reviewed, UniProtKB/Swiss-Prot A7GUA1 (GLGB_BACCN)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Bcer98_3504

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000083065
Sites
	Active site	204	1	By similarity
	Active site	239	1	By similarity
	Active site	244	1	By similarity
	Active site	307	1	By similarity
	Active site	309	1	By similarity
	Active site	352	1	By similarity
	Active site	419	1	By similarity
	Active site	420	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GUA1-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: E77F40A241FE1BD2	FASTA	645	76,146
	10 20 30 40 50 60 MRAIHCLEER LEQFHTEKCY ESYQIFGAHL AVENGVHGVR FTVWAPRAKN LSVVGDFNEW 70 80 90 100 110 120 NEKQHRMQKV TEAGIWSLFI PQIEEKEIYK YAIETINGEV ILKADPYATY AEVRPNTASV 130 140 150 160 170 180 ILDIEGYEWN DKNWFRKKKK KSVYKEAMAI YELHFGSWKK KEDGSLYSYR EMAEELIPYM 190 200 210 220 230 240 ANHHFTHIEI MPLVEHPYDR SWGYQGTGYY AVTSRFGTPH DFMYFVDECH KYGIGVILDW 250 260 270 280 290 300 VPGHFCKDAH GLYLFDGTPT YEYRDLDVQE NRVWGTANFD LGKREVRNFL ISNALFWMKY 310 320 330 340 350 360 YHIDGFRVDA VANMLYWEKE GKAQSNEYAV SFLRELNEAV FAEDGEFLMT AEDSTAWPLV 370 380 390 400 410 420 TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHEK MTFSLLYAYS ENFILPLSHD 430 440 450 460 470 480 EVVHGKKSLL NKMPGNYWEK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 490 500 510 520 530 540 NLHEFEMHRH MHDYFKELIA LYKRSKPLWQ LDYSHEGFQW IDADNKEQSI FSFIRKGDRE 550 560 570 580 590 600 DDVLIVICNF TSIVYEKYKV GVPEFQYYNE ILNSDAIAYG GSGRINKKRL KSISQPYHNQ 610 620 630 640 TAHVEITIPP FGVSILRPVK MRKGSKKQDG KKAELRSNAT SRRKR

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS23709.1.
RefSeq	YP_001376704.1.
3D structure databases
SMR	A7GUA1. Positions 21-620.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GUA1.
Genome annotation databases
GeneID	5347533.
GenomeReviews	Gene locus Bcer98_3504 in contig CP000764_GR.
KEGG	bcy:Bcer98_3504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACCN

Accession

Primary (citable) accession number: A7GUA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)