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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateUniRule annotation1
Binding sitei192SubstrateUniRule annotation1
Metal bindingi198ManganeseUniRule annotation1
Binding sitei198ATPUniRule annotation1
Binding sitei198SubstrateUniRule annotation1
Metal bindingi217Manganese; via tele nitrogenUniRule annotation1
Binding sitei217ATPUniRule annotation1
Metal bindingi254ManganeseUniRule annotation1
Binding sitei282ATPUniRule annotation1
Binding sitei319ATPUniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Binding sitei444ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi233 – 241ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:Bcer98_3438
OrganismiBacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Taxonomic identifieri315749 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002300 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000809901 – 528Phosphoenolpyruvate carboxykinase [ATP]Add BLAST528

Interactioni

Protein-protein interaction databases

STRINGi315749.Bcer98_3438.

Structurei

3D structure databases

ProteinModelPortaliA7GU41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7GU41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVNVQIGL HELLNGSNAQ IQLSVPQLVE KVLMRNEGKL TSTGAVSAST
60 70 80 90 100
GKYTGRSPKD KFIVKEPSVA NKIAWGPVNQ PISEERFNKL YTKVLEYLKE
110 120 130 140 150
KEELFVFKGF AGADRNYRLP IQVVNEYAWH NLFVHQLFIR PTEEELANHN
160 170 180 190 200
APFTIVSAPN FKADPAVDGT NSEAFIIVSF EKRIVLIGGT EYAGEMKKSI
210 220 230 240 250
FSIMNFLLPE QDILSMHCSA NVGEEGDVAL FFGLSGTGKT TLSADPHRKL
260 270 280 290 300
IGDDEHGWSD NGVFNIEGGC YAKCINLSHE KEPQIFDAIK FGSVLENVIV
310 320 330 340 350
DDKTRIADYS DTALTENTRA AYPIDAIDNI VLPSVAGHPN TIIFLTADAS
360 370 380 390 400
GVLPPISKLS KEQAMYHFLS GYTSKLAGTE RGVTSPQATF STCFGSPFLP
410 420 430 440 450
LDASRYAEML GEKIEKHDAK VFLVNTGWTG GEYGVGKRMN LAYTRAMVQA
460 470 480 490 500
ALNGELDKVE TVKHDIFGLD VPLHVPSVPD EVLMPEQTWA DQAAYKQKAI
510 520
ELANQFKENF KKFDNVSKDI INLGGPTA
Length:528
Mass (Da):58,078
Last modified:September 11, 2007 - v1
Checksum:iD5880E64720390F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA. Translation: ABS23649.1.
RefSeqiWP_012095897.1. NC_009674.1.

Genome annotation databases

EnsemblBacteriaiABS23649; ABS23649; Bcer98_3438.
KEGGibcy:Bcer98_3438.
PATRICi18935526. VBIBacCyt128034_3604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA. Translation: ABS23649.1.
RefSeqiWP_012095897.1. NC_009674.1.

3D structure databases

ProteinModelPortaliA7GU41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315749.Bcer98_3438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS23649; ABS23649; Bcer98_3438.
KEGGibcy:Bcer98_3438.
PATRICi18935526. VBIBacCyt128034_3604.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_BACCN
AccessioniPrimary (citable) accession number: A7GU41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: November 30, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.