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A7GU19 (SYL_BACCN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bcer98_3415
OrganismBacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]
Taxonomic identifier315749 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length802 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 802802Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074824

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7GU19 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: BE0656D9D4109E08

FASTA80291,593
        10         20         30         40         50         60 
MSFNHQEIEK KWQAYWEENK TFRTPDETDK PKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNSPAEFT EKNINTFRNQ IKSLGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPN YYKWTQWIFL KLFEKGLAYV DEIPVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVERRPMKQW MLKITAYADR LLEDLDELDW PESLKDMQRN WIGRSEGAEV HFNIDGTDKK 

       250        260        270        280        290        300 
FTIFTTRPDT LFGATYCVLA PEHALVAEIT TEDQKGAVEA YIDVVKSKSD LERTELAKEK 

       310        320        330        340        350        360 
TGVFTGAYAI NPVNGEKLPI WIADYVLASY GTGAVMAVPA HDERDYEFAK TFDLPMKEVV 

       370        380        390        400        410        420 
KGGDITKEAY TADGEHIDSA FLNGLNKEEA IAKMIEWLEV TGAGNQKVTY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGTMTAV KEEELPLVLP KTDNIRPSGT GESPLANIDE WVNVVDPETG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSCWYY LRYIDPNNNE ALVDPEKAKQ WLPVDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD IGVVPTKEPF QQLFNQGMIL GENNEKMSKS KGNVVNPDDI VASHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIAWSENGL DGARRFLDRV WRLFVQENGE LSEKITDAPN KELEKAYHQT 

       670        680        690        700        710        720 
VKKVTEDYEE LHFNTAISQM MMFINDAYKA ETLPKEYVEG FVKLLAPVAP HIAEELWSKL 

       730        740        750        760        770        780 
GYNETITYAS WPTFDESKLV EDEVEIVVQV MGKVRAKLKM KKDASKEEME QLALEEVKEQ 

       790        800 
IEGKTVRKVI VVPGKLVNIV AN 

« Hide

References

[1]"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity."
Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A.
Chem. Biol. Interact. 171:236-249(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NVH 391-98.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000764 Genomic DNA. Translation: ABS23627.1.
RefSeqYP_001376622.1. NC_009674.1.

3D structure databases

ProteinModelPortalA7GU19.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315749.Bcer98_3415.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS23627; ABS23627; Bcer98_3415.
GeneID5346829.
KEGGbcy:Bcer98_3415.
PATRIC18935480. VBIBacCyt128034_3581.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHELEVYT.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBCYT315749:GH2A-3525-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACCN
AccessionPrimary (citable) accession number: A7GU19
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries