A7GTV6 (MURC_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Bcer98_3349

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000074729
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7GTV6 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 8C2356DEE4C67BA2	FASTA	436	49,373
	10 20 30 40 50 60 MTVYHFVGIK GTGMSSLAQI LHDMKNTVQG SDYEKRFFTQ VALEKRGISI LPFDKNNIKE 70 80 90 100 110 120 GQVIIAGNAF PDTHEEIMAA KELNIPVHRY HHFLGNLMSQ YTSVAVTGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQGANPTS YLIGDGTGHG VENSKYFAFE ACEYRRHFLS YYPDYAIMTN IDFDHPDYFA 190 200 210 220 230 240 DINDVFHAFQ EMALQVKKGI IACGDDEELQ KIQAKVPVIF YGFGEDNDFQ ARNIQKRTDG 250 260 270 280 290 300 TVFDVFVRNT YYETFKITGY GNHSVLNALA VIALCHYENI DVEVVKHQLT TFEGVKRRFN 310 320 330 340 350 360 EKTVRDQVII DDYAHHPTEI NATIEAAHQK YPEREIIAIF QPHTFSRTET FLDEFAESLS 370 380 390 400 410 420 KADQVYLCDI FGSARENKGE LTIEDLQKRI DGAELITDTT TDILKKHKNG VLIFMGAGDI 430 QKFEAAYIKE VQVAEK

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS23564.1.
RefSeq	YP_001376559.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GTV6.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GTV6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000038060; EBBACP00000037111; EBBACG00000038051.
GeneID	5343882.
GenomeReviews	Gene locus Bcer98_3349 in contig CP000764_GR.
KEGG	bcy:Bcer98_3349.
PATRIC	18935342. VBIBacCyt128034_3512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
GeneTree	EBGT00050000000630.
HOGENOM	HBG476594.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_3349-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACCN

Accession

Primary (citable) accession number: A7GTV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program