A7GTK8 (SYN_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Bcer98_3247

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000081844

Sequences

	Sequence		Length	Mass (Da)
	A7GTK8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: D6CAE7361F8F1514	FASTA	463	53,415
	10 20 30 40 50 60 MENTLVKSLY RETDKYVDQK VQVSGWIRNL RDSKVFGFIE LNDGSFFKSV QIVFDTELDN 70 80 90 100 110 120 FKEITKLPLS SSIRVEGKFI ATPTAKQPFE IKAEKIEIEG LSDSDYPLQK KRHTFEYLRT 130 140 150 160 170 180 IAHLRPRTNA FSATFRVRSI AAYAIHKFFQ ERGFVHVHTP IITGSDTEGA GEMFRVTTHD 190 200 210 220 230 240 MNNLPKGEDG QVDDSKDFFG RETNLTVSGQ LPAEAYALAF RDVYTFGPTF RAENSNTTRH 250 260 270 280 290 300 AAEFWMVEPE IAFAELEDVM DLTEDMLKYA MKYVLEHAPE EMEFFNKFVD KTVLERMNNV 310 320 330 340 350 360 INSDFGRITY TEAIKVLQES GADFKYPVEW GIDLQTEHER YLSEQVFKRP VFVTDYPKDI 370 380 390 400 410 420 KAFYMRMNDD GKTVAATDLL VPGIGELIGG SQREERMDVL VDRIKELGMK EEDYWWYLEL 430 440 450 460 RKYGGTKHAG FGLGFERFLM YITGMGNIRD VIPFPRTPGS AEF

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS23466.1.
RefSeq	YP_001376461.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GTK8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GTK8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000038716; EBBACP00000037767; EBBACG00000038707.
GeneID	5343770.
GenomeReviews	Gene locus Bcer98_3247 in contig CP000764_GR.
KEGG	bcy:Bcer98_3247.
PATRIC	18935136. VBIBacCyt128034_3409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
GeneTree	EBGT00050000000858.
HOGENOM	HBG745843.
OMA	AIHRFFH.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_3247-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACCN

Accession

Primary (citable) accession number: A7GTK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program