A7GT84 (SYD_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Bcer98_3118

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000074690

Sequences

	Sequence		Length	Mass (Da)
	A7GT84 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: C784E1F4C2E2CA7A	FASTA	589	66,065
	10 20 30 40 50 60 MAERTHACGR VTVEAIGQTV QLKGWVQKRR DLGGLIFIDL RDRTGIVQVV FSPETSKEAL 70 80 90 100 110 120 EVAETIRGEY VLHVEGTVVA RGAGAINENM ATGQIEVQAT KVTVLNAAKT TPIIIADDTD 130 140 150 160 170 180 ASEDVRLKYR YLDLRRPVMY NTFKMRHDVT KTIRNFLDTE EFLEVETPIL TKSTPEGARD 190 200 210 220 230 240 YLVPSRVHDG EFYALPQSPQ LFKQLLMVGG FERYYQVARC FRDEDLRADR QPEFTQIDIE 250 260 270 280 290 300 ASFLTQEEIL DMMERMMTKV MKDVKGVEIS VPFPRMTYAD AMARYGSDKP DTRFEMELTD 310 320 330 340 350 360 LSEFAAGCGF KVFTGAVENG GQVKAINAKG AASKYSRKDI DALTEFVKVY GAKGLAWLKV 370 380 390 400 410 420 EEDGLKGPIA KFFNEEEANV IMTTLEASAG DLLLFVADKK SVVADSLGAL RLRLGKELEL 430 440 450 460 470 480 IDENKFNFLW VTDWPLLEYD EEANRYFAAH HPFTMPFRED VELLETAPEK ARAQAYDLVL 490 500 510 520 530 540 NGYELGGGSL RIYERDVQEK MFKALGFTQE EAREQFGFLL EAFEYGTPPH GGIALGLDRL 550 560 570 580 VMLLAGRTNL RDTIAFPKTA SASCLLTDAP SPVAGAQLEE LHLKLNVKE

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS23342.1.
RefSeq	YP_001376337.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GT84.
SMR	A7GT84. Positions 4-585.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GT84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000038158; EBBACP00000037209; EBBACG00000038149.
GeneID	5346047.
GenomeReviews	Gene locus Bcer98_3118 in contig CP000764_GR.
KEGG	bcy:Bcer98_3118.
PATRIC	18934866. VBIBacCyt128034_3276.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
GeneTree	EBGT00050000000858.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_3118-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACCN

Accession

Primary (citable) accession number: A7GT84

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program