A7GSJ5 (DXS_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Bcer98_2870

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000079081

Sequences

	Sequence		Length	Mass (Da)
	A7GSJ5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 2CB9FFFCA3EFD11B	FASTA	630	69,600
	10 20 30 40 50 60 MDLTQIQNPS FLKDMSISEL EELSEDIRKF LIEELSQTGG HIAPNLGVVE LTIALHTLFD 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKIL TGRAKEFRTL RQYKGLCGFP KRCESEHDVW ETGHSSTSLS 130 140 150 160 170 180 AAMGMALARD LKKTDEYVIP IIGDGALTGG MALEALNHIG HEKTDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGALHNVLGR LRTAGKYQWV KDELEYILKK IPAVGGKVAA TAEKIKDSLK YLLVSGVFFE 250 260 270 280 290 300 ELGFTYLGPV DGHDYEKLFE TLQYAKKTKG PVLVHVITKK GKGYKPAESD VIGTWHGTGP 310 320 330 340 350 360 YKIESGDFVK PKEVAPAWSA VVSETVRKLA RVDERIVAIT PAMPVGSKLE KFHQEFPNRM 370 380 390 400 410 420 IDVGIAEQHA TTMAAGMATQ GMKPFLAIYS TFLQRAYDQV VHDICRQNLN VFIGIDRSGL 430 440 450 460 470 480 VGADGETHQG VFDIAFLRHL PNIVLMMPKD ENEGQHLVYT AMQYEDGPIA LRYPRGNGLG 490 500 510 520 530 540 VPMDDEFKVI PIGTWETLRE GTQAAIVTFG TTIPMAMEAA ERLGKAGVSV KVVNARFIKP 550 560 570 580 590 600 MDEAYLHDLL GKNIPILTIE EACLIGGFGS GVMEFAAEHG YHSALIERMG IPDYFIEHGS 610 620 630 VTKLLEEIGL TTDAVVDRIH TMIPSKQKRA

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS23103.1.
RefSeq	YP_001376098.1. NC_009674.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7GSJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000039302; EBBACP00000038353; EBBACG00000039293.
GeneID	5343748.
GenomeReviews	Gene locus Bcer98_2870 in contig CP000764_GR.
KEGG	bcy:Bcer98_2870.
PATRIC	18934354. VBIBacCyt128034_3021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
GeneTree	EBGT00070000031844.
HOGENOM	HBG571647.
OMA	EDGRTHH.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_2870-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACCN

Accession

Primary (citable) accession number: A7GSJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program