A7GS30 (METE_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bcer98_2704

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000077110
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GS30 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 9925A328AEBA14F4	FASTA	762	87,599
	10 20 30 40 50 60 MALHTSNLGY PRIGLQREWK KTLEAFWAQK IDEQQLITTM KEIHLEHLNV QKEKGIDFIP 70 80 90 100 110 120 IGDFTYYDHV LDTAYMLGFI PSRFSNFTSY LDIYFAMARG SKDHVASEMT KWFNTNYHYI 130 140 150 160 170 180 VPEYEEGLNI SLKDNRPLRL YEEAKRELGI DGKPVILGPY TFLKLAKGYT QDQFPTILHK 190 200 210 220 230 240 LIAPYVQLLT ELHKAGARLI QIDEPIFVSL TKEEMIEAKK LYEAIQKEVP SANLILQTYF 250 260 270 280 290 300 DSVEENYTEL ITFPVSGIGL DFVHGKEGNI KSIVEHGFPA EKTLAIGCID GRNIWRANLD 310 320 330 340 350 360 EVFALFETLK AHIEPKDWII QPSCSLLHTP VDKTEETHLS PELFDALAFA NQKLEELTLI 370 380 390 400 410 420 KRGLSEGVNS IEIEITTYRN SHEAVRSSAA RNRKDVQTAR ASLKEEDFSR PLPFEQRYDL 430 440 450 460 470 480 QQKALQLPLL PTTTIGSFPQ TPEVRQTRKQ WRNGEITNEQ YEHFIEQETE KWIRHQENIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE RLAGFSFTKN GWVQSYGSRC VKPPIIFGDV AFISDMTVKE 550 560 570 580 590 600 TVYAQSLTNK VVKGMLTGPV TILNWSFVRN DLPRKEVCYQ IALALRHEIE RLESSGIRII 610 620 630 640 650 660 QVDEPALREG MPLKEKDWDS YIEWAVQSFR LATASVANET QIHTHMCYSN FEDIVDAIRA 670 680 690 700 710 720 LDADVISIET SRSHGEFIHT LEQTTYEKGI GLGVYDIHSP RVPSKEEMYT IVEQSLKVCN 730 740 750 760 PKYFWINPDC GLKTRRTEEV LPALEHMVEA AKEARTLLKT NA

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS22938.1.
RefSeq	YP_001375933.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GS30.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GS30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000039130; EBBACP00000038181; EBBACG00000039121.
GeneID	5345245.
GenomeReviews	Gene locus Bcer98_2704 in contig CP000764_GR.
KEGG	bcy:Bcer98_2704.
PATRIC	18934018. VBIBacCyt128034_2853.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
GeneTree	EBGT00050000001686.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_2704-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACCN

Accession

Primary (citable) accession number: A7GS30

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program