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A7GRK7 (PYRE_BACCN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:Bcer98_2531
OrganismBacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]
Taxonomic identifier315749 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_1000085537

Regions

Region120 – 12895-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site9415-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site9815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10015-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1241Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
A7GRK7 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 91205BCC63BF60BE

FASTA21022,760
        10         20         30         40         50         60 
MKKEIASHLL EIGAVFLQPN DPFTWSSGMK SPIYCDNRLT LSYPKVRQAI AAGLEELIKE 

        70         80         90        100        110        120 
HFPTVEVIAG TATAGIAHAA WVSDRMNLPM CYVRSKAKGH GKGNQIEGKA EKGQKVVVVE 

       130        140        150        160        170        180 
DLISTGGSAI TCVEALREAG CEVLGIVSIF TYELEAGKEK LAAANVASYS LSDYSTLTQV 

       190        200        210 
AEEKGMIGQA EMKKLQEWRK NPADEAWITA

« Hide

References

[1]"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity."
Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A.
Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NVH 391-98.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000764 Genomic DNA. Translation: ABS22765.1.
RefSeqYP_001375760.1. NC_009674.1.

3D structure databases

ProteinModelPortalA7GRK7.
SMRA7GRK7. Positions 1-204.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7GRK7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000039365; EBBACP00000038416; EBBACG00000039356.
GeneID5343385.
GenomeReviewsGene locus Bcer98_2531 in contig CP000764_GR.
KEGGbcy:Bcer98_2531.
PATRIC18933660. VBIBacCyt128034_2674.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0461.
GeneTreeEBGT00050000002725.
HOGENOMHBG404341.
OMALPMTYVR.
ProtClustDBPRK00455.

Enzyme and pathway databases

BioCycBCER315749:BCER98_2531-MONOMER.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_BACCN
AccessionPrimary (citable) accession number: A7GRK7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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