A7GRG3 (A7GRG3_BACCN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABS22721.1
EC=5.99.1.2 EMBL ABS22721.1

Gene names

Ordered Locus Names:

Bcer98_2485

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP] EMBL ABS22721.1

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A7GRG3 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 82997C823F4D912C	FASTA	692	78,985
	10 20 30 40 50 60 MSDYLVIVES PSKAKTIEKY LGKKYKVVAS MGHVRDLPKS QMGIEVKNNF TPKYITIRGK 70 80 90 100 110 120 GPVLKELKSA AKKAKKVYLA ADPDREGEAI AWHLANTLNV DVESDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRAINM DLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVRLIIERER 190 200 210 220 230 240 EIQNFVPEEF WTIKTEFVKG KETFEAAFYG VNGEKVQLTN EAQVKDIIEK IKDNAFSVED 250 260 270 280 290 300 VTKKERKRNP ALPFTTSSLQ QEAARKLNMR AKKTMMLAQQ LYEGIDIGKQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISETA QEEARSYIAD AFGSEYIGTG KKKETKKANA QDAHEAIRPT SVMRKPDELK 370 380 390 400 410 420 SFLSRDQYRL YKLIWERFVA SQMASAIMDT VTAKLINNDV QFRASGSVVK FPGFMKVYVE 430 440 450 460 470 480 SKDDGAEEKD KMLPPLEVGE TVFSKDVEPK QHFTQPPPRY TEARLVRTLE ELGIGRPSTY 490 500 510 520 530 540 VPTLETIQKR GYVALDNKRF VPTELGEIVT ELMLEFFPEI INIEFTANME QNLDEIEEGK 550 560 570 580 590 600 ANWVTIVDDF YTEFAPRLEK AEKEMREIEI KDEPAGEDCE LCGHPMVFKM GRYGKFMACS 610 620 630 640 650 660 NFPDCRNTKP IVKEIGVTCP KCEKGQIIER RSNNKKRLFY GCSTYPECDF VSWDKPIGRK 670 680 690 CPKCEGMLIE KKLKKGVQVQ CISCDYEEEQ QM

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS22721.1.
RefSeq	YP_001375716.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GRG3.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GRG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000037112; EBBACP00000036163; EBBACG00000037103.
GeneID	5345967.
GenomeReviews	Gene locus Bcer98_2485 in contig CP000764_GR.
KEGG	bcy:Bcer98_2485.
PATRIC	18933570. VBIBacCyt128034_2629.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00070000032133.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A7GRG3_BACCN

Accession

Primary (citable) accession number: A7GRG3

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)