A7GP90 (TYSY_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Bcer98_1639

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cytotoxicus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP-Rule MF_00008		PRO_1000073867
Sites
	Active site	200	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GP90 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: CD855B461136241F	FASTA	318	36,810
	10 20 30 40 50 60 MKHAEYEYLN LCRHVMEHGT KKEDRTGTGT VSVFGYQMRF DLSKGFPLLT TKRVPFRLVA 70 80 90 100 110 120 SELLWFMKGD TNIRYLLQHN NNIWNEWAFK NWVESDEYKG PDMTNFGLRS QEDEAFKTQY 130 140 150 160 170 180 DEQMEIFKKN VLEDDEFARK YGYLGDVYGK QWRAWKTAAG ETIDQLKDVI EMIKKTPDSR 190 200 210 220 230 240 RLIVSAWNPE DVPNMALPPC HTLFQFYVAD GKLSCQLYQR SGDIFLGIPF NIASYSLLTH 250 260 270 280 290 300 LIAHECGLAV GEFVHTIGDA HIYTNHFEQV KKQLAREPRP FPTLKLNSDV KSVFDFEMGD 310 LTIEGYDPHP AIKAPVAV

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS21948.1.
RefSeq	YP_001374943.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GP90.
SMR	A7GP90. Positions 5-318.
ModBase	Search...
Protein-protein interaction databases
STRING	315749.Bcer98_1639.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS21948; ABS21948; Bcer98_1639.
GeneID	5347186.
KEGG	bcy:Bcer98_1639.
PATRIC	18931750. VBIBacCyt128034_1719.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	BCYT315749:GH2A-1786-MONOMER.
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 2 hits.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACCN

Accession

Primary (citable) accession number: A7GP90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program