Reviewed, UniProtKB/Swiss-Prot A7GP90 (TYSY_BACCN)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Bcer98_1639

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP MF_00008		PRO_1000073867
Sites
	Active site	200	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GP90-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: CD855B461136241F	FASTA	318	36,810
	10 20 30 40 50 60 MKHAEYEYLN LCRHVMEHGT KKEDRTGTGT VSVFGYQMRF DLSKGFPLLT TKRVPFRLVA 70 80 90 100 110 120 SELLWFMKGD TNIRYLLQHN NNIWNEWAFK NWVESDEYKG PDMTNFGLRS QEDEAFKTQY 130 140 150 160 170 180 DEQMEIFKKN VLEDDEFARK YGYLGDVYGK QWRAWKTAAG ETIDQLKDVI EMIKKTPDSR 190 200 210 220 230 240 RLIVSAWNPE DVPNMALPPC HTLFQFYVAD GKLSCQLYQR SGDIFLGIPF NIASYSLLTH 250 260 270 280 290 300 LIAHECGLAV GEFVHTIGDA HIYTNHFEQV KKQLAREPRP FPTLKLNSDV KSVFDFEMGD 310 LTIEGYDPHP AIKAPVAV

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000764 Genomic DNA. Translation: ABS21948.1.
RefSeq	YP_001374943.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7GP90.
Genome annotation databases
GeneID	5347186.
GenomeReviews	Gene locus Bcer98_1639 in contig CP000764_GR.
KEGG	bcy:Bcer98_1639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LGPVYGH.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 2 hits.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACCN

Accession

Primary (citable) accession number: A7GP90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)