A7GNY4 (NAMA_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	Bcer98_1529

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	NADPH dehydrogenase HAMAP MF_01614		PRO_0000323520
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GNY4 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 65ED5C561EBC61F0	FASTA	346	38,479
	10 20 30 40 50 60 MNTQLFSPFT IQNVTLKNRI VMSPMCMYSS ENEDGKVTNF HLIHYGTRAM GQVGLVMLEA 70 80 90 100 110 120 TAVAAEGRIS NKDLGIWNDE HIEGLQKTVS FIKEHDSKAA IQLAHAGRKA ELHTDPVAPS 130 140 150 160 170 180 AIPFNDKMKV PVAMSKEQIQ DTITAFQKAA IRSKQAGFDV IELHGAHGYL INEFLSPLSN 190 200 210 220 230 240 KRTDEYGGTA ENRYRFLREI IHAVKEVWEG PLFVRISADD YHPEGLTVND YVQFAKWMKE 250 260 270 280 290 300 QGVDLIDCSS GAVVPAHIDV YPGYQVKYAK HLKEHAQIAT GAVGLITSGV QAEQILVNSE 310 320 330 340 ADLIFVGREL LRNPYFPRTA ANELGFELKD PHQYSRAPGK IANTSK

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS21842.1.
RefSeq	YP_001374837.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GNY4.
SMR	A7GNY4. Positions 4-337.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GNY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000039040; EBBACP00000038091; EBBACG00000039031.
GeneID	5345792.
GenomeReviews	Gene locus Bcer98_1529 in contig CP000764_GR.
KEGG	bcy:Bcer98_1529.
PATRIC	18931532. VBIBacCyt128034_1610.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1902.
GeneTree	EBGT00050000002219.
HOGENOM	HBG583461.
OMA	WVEDGWN.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_1529-MONOMER.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00356.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACCN

Accession

Primary (citable) accession number: A7GNY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program