A7GNV5 (KHSE_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	Bcer98_1498

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_1000080117
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7GNV5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: CD9B9986427745F7	FASTA	297	32,479
	10 20 30 40 50 60 MIPFKIRVPA STANIGPGFD SVGMALSLYL EVIVKEEVPH WYVIHPFDQS VPNDDSNLII 70 80 90 100 110 120 STALQVCPSL PSHIIEVISN IPLTRGLGSS ASAIVAGIEL ANQLGALHLT TDEKIHLATK 130 140 150 160 170 180 FEGHPDNVAA SILGGTVIGA IDEGHVSVVR IESKELGVIS VIPDEELNTN ESRFVLPETF 190 200 210 220 230 240 SFHHAVRASS ISNVLVAALC EKKWEIVGEM MERDLFHEPY RSQLVPLLPS VREYAKRFGA 250 260 270 280 290 YGTALSGAGP SLFILTPYEN RQEIAEQLMK VFPKIEVCEL EIDYEGVVVN MDISVNR

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS21813.1.
RefSeq	YP_001374808.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GNV5.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GNV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000038778; EBBACP00000037829; EBBACG00000038769.
GeneID	5346192.
GenomeReviews	Gene locus Bcer98_1498 in contig CP000764_GR.
KEGG	bcy:Bcer98_1498.
PATRIC	18931464. VBIBacCyt128034_1576.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000000874.
HOGENOM	HBG646290.
OMA	DRLHQPY.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_1498-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACCN

Accession

Primary (citable) accession number: A7GNV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program