Reviewed, UniProtKB/Swiss-Prot A7GNQ7 (ILVD_BACCN)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Bcer98_1446

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000073965
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7GNQ7-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: F8D3E37706AD6A8E	FASTA	557	60,085
	10 20 30 40 50 60 MRSDMIKKGF DKAPHRSLLK ATGLKDEDFH KPFVAICNSF IEIIPGHKHL NEFGRLVKEA 70 80 90 100 110 120 VRAAGMVPFE FNTIGVDDGI AMGHIGMRYS LPSREIIADS VETVVNAHWF DGMICIPNCD 130 140 150 160 170 180 KITPGMMMAA MRINIPTVFV SGGPMAAGKT SKGEVVDLSS VFEGVGAYQS GKISEEELKD 190 200 210 220 230 240 IEDHGCPSCG SCSGMFTANS MNCLCEVLGL ALPGNGSILA TDSRREQLIQ RAAESLKILM 250 260 270 280 290 300 ERDIKPRDIV TEEAIDDAFA LDMAMGGSTN TVLHTLAIAQ EAGLDYDMNR IDAVSRKVPH 310 320 330 340 350 360 LCKVSPASNW HMEDIDRAGG MSAILKELSR KEGVLHFDRI TVTGKTLREN IADAEIQDKE 370 380 390 400 410 420 VIHSLENPHS QEGGLRILKG NLAKDGAVIK SGATEVKRFE GPCVIFNSQD EALAGIMLGK 430 440 450 460 470 480 VKKGDVVVIR YEGPRGGPGM PEMLAPTSAI AGMGLGADVA LLTDGRFSGA SRGISVGHIS 490 500 510 520 530 540 PEAAAGGEIA LLQQGDIVCI DVEERLLEVK VSDEELAKRR KEWKRPEPKV KTGWLGRYAQ 550 MVTSANTGAV LKMQNFD

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000764 Genomic DNA. Translation: ABS21765.1.
RefSeq	YP_001374760.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7GNQ7.
Genome annotation databases
GeneID	5344108.
GenomeReviews	Gene locus Bcer98_1446 in contig CP000764_GR.
KEGG	bcy:Bcer98_1446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACCN

Accession

Primary (citable) accession number: A7GNQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)