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Protein

CCA-adding enzyme

Gene

cca

Organism
Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26ATP or CTP; via amide nitrogenUniRule annotation1
Binding sitei29ATP or CTPUniRule annotation1
Metal bindingi39MagnesiumUniRule annotation1
Metal bindingi41MagnesiumUniRule annotation1
Binding sitei110ATP or CTPUniRule annotation1
Binding sitei153ATP or CTPUniRule annotation1
Binding sitei156ATP or CTPUniRule annotation1
Binding sitei159ATP or CTPUniRule annotation1
Binding sitei162ATP or CTPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
Biological processRNA repair, tRNA processing
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:Bcer98_1260
OrganismiBacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Taxonomic identifieri315749 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000858211 – 397CCA-adding enzymeAdd BLAST397

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi315749.Bcer98_1260

Structurei

3D structure databases

ProteinModelPortaliA7GN74
SMRiA7GN74
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D4J Bacteria
COG0617 LUCA
HOGENOMiHOG000253346
KOiK00974
OMAiWKNSNAM
OrthoDBiPOG091H022U

Family and domain databases

CDDicd05398 NT_ClassII-CCAase, 1 hit
HAMAPiMF_01263 CCA_bact_type3, 1 hit
InterProiView protein in InterPro
IPR032810 CCA-adding_enz_C
IPR023068 CCA-adding_enz_firmicutes
IPR002646 PolA_pol_head_dom
IPR032828 PolyA_RNA-bd
PfamiView protein in Pfam
PF01743 PolyA_pol, 1 hit
PF12627 PolyA_pol_RNAbd, 1 hit
PF13735 tRNA_NucTran2_2, 1 hit

Sequencei

Sequence statusi: Complete.

A7GN74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFKRAGAI IETLKEHGHE AYFVGGSVRD FIIDRPIGDI DIATSALPEE
60 70 80 90 100
VMKLFPKHVP VGLEHGTVIV LQDGEPYEVT TFRTESDYED FRRPSSVQFV
110 120 130 140 150
RSLEEDLKRR DFTMNAIAMN EDGEIIDLFG GQEAIQKREI VTVGNAAERF
160 170 180 190 200
QEDALRMMRG IRFVSTLGFS LEEKTECAIK RYGHLLEHIA IERITVEFEK
210 220 230 240 250
LLTGPYCVKG LQKLVETKLF MHLPYLQMSE EKILKAAEYN WESFETEIEA
260 270 280 290 300
WAFFLSCIGE EHPSVFLRQW KFSNKKIKEI VAVLLAIRTR KTKEWDAVFL
310 320 330 340 350
YQTGVQIALM AERVYQVMIE EYNMSTVSEV QRLFDSLPIQ KRQEMNVSGN
360 370 380 390
DLLSWTDKTP GPWVAEVLQK IEEEILQKRL ENEKEAIRGW IEECNLL
Length:397
Mass (Da):46,056
Last modified:September 11, 2007 - v1
Checksum:i370F6E531E534FDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA Translation: ABS21582.1
RefSeqiWP_012093749.1, NC_009674.1

Genome annotation databases

EnsemblBacteriaiABS21582; ABS21582; Bcer98_1260
GeneIDi33896609
KEGGibcy:Bcer98_1260

Entry informationi

Entry nameiCCA_BACCN
AccessioniPrimary (citable) accession number: A7GN74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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