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Protein

CCA-adding enzyme

Gene

cca

Organism
Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261ATP or CTP; via amide nitrogenUniRule annotation
Binding sitei29 – 291ATP or CTPUniRule annotation
Metal bindingi39 – 391MagnesiumUniRule annotation
Metal bindingi41 – 411MagnesiumUniRule annotation
Binding sitei110 – 1101ATP or CTPUniRule annotation
Binding sitei153 – 1531ATP or CTPUniRule annotation
Binding sitei156 – 1561ATP or CTPUniRule annotation
Binding sitei159 – 1591ATP or CTPUniRule annotation
Binding sitei162 – 1621ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciBCYT315749:GH2A-1372-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:Bcer98_1260
OrganismiBacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Taxonomic identifieri315749 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002300 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397CCA-adding enzymePRO_1000085821Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi315749.Bcer98_1260.

Structurei

3D structure databases

ProteinModelPortaliA7GN74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D4J. Bacteria.
COG0617. LUCA.
HOGENOMiHOG000253346.
KOiK00974.
OMAiGNFKACK.
OrthoDBiEOG65QWJN.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7GN74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFKRAGAI IETLKEHGHE AYFVGGSVRD FIIDRPIGDI DIATSALPEE
60 70 80 90 100
VMKLFPKHVP VGLEHGTVIV LQDGEPYEVT TFRTESDYED FRRPSSVQFV
110 120 130 140 150
RSLEEDLKRR DFTMNAIAMN EDGEIIDLFG GQEAIQKREI VTVGNAAERF
160 170 180 190 200
QEDALRMMRG IRFVSTLGFS LEEKTECAIK RYGHLLEHIA IERITVEFEK
210 220 230 240 250
LLTGPYCVKG LQKLVETKLF MHLPYLQMSE EKILKAAEYN WESFETEIEA
260 270 280 290 300
WAFFLSCIGE EHPSVFLRQW KFSNKKIKEI VAVLLAIRTR KTKEWDAVFL
310 320 330 340 350
YQTGVQIALM AERVYQVMIE EYNMSTVSEV QRLFDSLPIQ KRQEMNVSGN
360 370 380 390
DLLSWTDKTP GPWVAEVLQK IEEEILQKRL ENEKEAIRGW IEECNLL
Length:397
Mass (Da):46,056
Last modified:September 11, 2007 - v1
Checksum:i370F6E531E534FDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA. Translation: ABS21582.1.
RefSeqiWP_012093749.1. NC_009674.1.

Genome annotation databases

EnsemblBacteriaiABS21582; ABS21582; Bcer98_1260.
KEGGibcy:Bcer98_1260.
PATRICi18930974. VBIBacCyt128034_1331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA. Translation: ABS21582.1.
RefSeqiWP_012093749.1. NC_009674.1.

3D structure databases

ProteinModelPortaliA7GN74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315749.Bcer98_1260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS21582; ABS21582; Bcer98_1260.
KEGGibcy:Bcer98_1260.
PATRICi18930974. VBIBacCyt128034_1331.

Phylogenomic databases

eggNOGiENOG4105D4J. Bacteria.
COG0617. LUCA.
HOGENOMiHOG000253346.
KOiK00974.
OMAiGNFKACK.
OrthoDBiEOG65QWJN.

Enzyme and pathway databases

BioCyciBCYT315749:GH2A-1372-MONOMER.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 22905 / CIP 110041 / 391-98 / NVH 391-98.

Entry informationi

Entry nameiCCA_BACCN
AccessioniPrimary (citable) accession number: A7GN74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: May 11, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.