Reviewed, UniProtKB/Swiss-Prot A7GMU0 (ILVC_BACCN)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Bcer98_1122

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000080617
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A7GMU0-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 242EB4E40EECFFDA	FASTA	336	36,751
	10 20 30 40 50 60 MAKVYYEKDV MVNVLKGKKV AIVGYGSQGH AHAQNLRDNN FDVVVGLRKG KSWEKAKEDG 70 80 90 100 110 120 FSVYSVAEAA AMADVVMVLL PDELQPEVYQ AEIESNLQSG NALVFAHGFN VHFNQVTPPE 130 140 150 160 170 180 DVDVFLVAPK GPGHLVRRTF TEGGAVPALF AVYQDATGTA TEKALSYADG IGATRAGVLE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GVTALVKAGF ETLVDAGYQP ELAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGLENMRYSV SDTAQWGDFV SGPRVVTEHT KKAMGDVLKE IQDGTFAKGW IAEHQAGRPH 310 320 330 FHAINAKENE HQIEVVGRQL REMMPFVKPK VKTGVK

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS21448.1.
RefSeq	YP_001374443.1.
3D structure databases
SMR	A7GMU0. Positions 3-327.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GMU0.
Genome annotation databases
GeneID	5344457.
GenomeReviews	Gene locus Bcer98_1122 in contig CP000764_GR.
KEGG	bcy:Bcer98_1122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HBG286525.
OMA	KDELTAG.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BACCN

Accession

Primary (citable) accession number: A7GMU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)