Reviewed, UniProtKB/Swiss-Prot A7GM94 (PRPE_BACCN)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
    EC=3.6.1.17
Alternative name(s):
    Ap4A hydrolase
    Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
      Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	Bcer98_0918

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity.
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity.
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: HAMAP nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_1000087460

Sequences

	Sequence		Length	Mass (Da)
	A7GM94-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 542FA0D5B65B890C	FASTA	246	28,015
	10 20 30 40 50 60 MKYDIMGDIH GCFQEFKTLT TKLGYNWDSG LPIHPEKRQL AFVGDITDRG PASLQMIEIV 70 80 90 100 110 120 WELVIKQKQA YYAPGNHCNK LYRFFLGRNV TIAHGLETTV AEYKALSSNE QAIIKHKFID 130 140 150 160 170 180 LYEQSPLYHI LDNNQLIVCH AGIRQDYIGR ADKKVQTFVL YGDITGEKHP DGSPVRRDWA 190 200 210 220 230 240 QNYTGNAWIV YGHTPVKEPR FVNHTVNIDT GAVFGGKLTG LRYPEMETIS VPSSLPFVPE KFRPIS

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000764 Genomic DNA. Translation: ABS21252.1.
RefSeq	YP_001374247.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5346460.
GenomeReviews	Gene locus Bcer98_0918 in contig CP000764_GR.
KEGG	bcy:Bcer98_0918.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7GM94. HCNKLYR.
Family and domain databases
HAMAP	MF_01443. [Tree]
InterPro	IPR004843. M-pesterase. IPR006186. T_phtase_apaH. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
ProtoNet	Search...

Entry information

Entry name

PRPE_BACCN

Accession

Primary (citable) accession number: A7GM94

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)