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Protein

Glycerol kinase

Gene

glpK

Organism
Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateUniRule annotation1
Binding sitei16ATPUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei266ATPUniRule annotation1
Binding sitei309ATP; via carbonyl oxygenUniRule annotation1
Binding sitei313ATP; via amide nitrogenUniRule annotation1
Binding sitei328ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14ATPUniRule annotation3
Nucleotide bindingi410 – 414ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycerol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:Bcer98_0807
OrganismiBacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Taxonomic identifieri315749 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000774091 – 496Glycerol kinaseAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230Phosphohistidine; by HPrUniRule annotation1

Post-translational modificationi

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Protein-protein interaction databases

STRINGi315749.Bcer98_0807

Structurei

3D structure databases

ProteinModelPortaliA7GLY7
SMRiA7GLY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni244 – 245Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HMR Bacteria
COG0554 LUCA
HOGENOMiHOG000222134
KOiK00864
OMAiWQDTRTQ
OrthoDBiPOG091H02FA

Family and domain databases

HAMAPiMF_00186 Glycerol_kin, 1 hit
InterProiView protein in InterPro
IPR000577 Carb_kinase_FGGY
IPR018485 Carb_kinase_FGGY_C
IPR018483 Carb_kinase_FGGY_CS
IPR018484 Carb_kinase_FGGY_N
IPR005999 Glycerol_kin
PfamiView protein in Pfam
PF02782 FGGY_C, 1 hit
PF00370 FGGY_N, 1 hit
PIRSFiPIRSF000538 GlpK, 1 hit
TIGRFAMsiTIGR01311 glycerol_kin, 1 hit
PROSITEiView protein in PROSITE
PS00933 FGGY_KINASES_1, 1 hit
PS00445 FGGY_KINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

A7GLY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METYILSLDQ GTTSSRAILF NKKGEIVHSA QKEFTQYFPK PGWVEHNAQE
60 70 80 90 100
IWGSILAVIA TCLSEADVKP EQIAGIGITN QRETAVVWEK ATGKPVYNAI
110 120 130 140 150
VWQSRQTAEI CEELKEKGYG DMVREKTGLL IDAYFSGTKV KWILDNVEGA
160 170 180 190 200
REKAERGELL FGTIDTWLVW KLSGGKAHVT DYSNASRTLM FNIHDLKWDD
210 220 230 240 250
ELLEILTVPK SMLPEVRPSS EVYGHTVDYH FFSQNVPIAG VAGDQQAALF
260 270 280 290 300
GQACFSEGMA KNTYGTGCFM LMNTGETAVN SNHGLLTTIA WGLNGKVNYA
310 320 330 340 350
LEGSIFVAGS AIQWLRDGMR MVNDASESEE YASRVESTDG VYVVPAFVGL
360 370 380 390 400
GTPYWDSEVR GAVFGVTRGT TKEHFIRATL ESLGYQTRDV LCAMEADSGI
410 420 430 440 450
ELKTLRVDGG AVKNNFLMQF QSDMLQVPVE RPMISETTAL GAAYLAGLAV
460 470 480 490
GYWESQEEIK AQWDMDRSFT PEMEKERSEE LYSGWKKAIE ATKAFK
Length:496
Mass (Da):55,071
Last modified:September 11, 2007 - v1
Checksum:iA7EC4F5C7FC6C9FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA Translation: ABS21145.1
RefSeqiWP_011983899.1, NC_009674.1

Genome annotation databases

EnsemblBacteriaiABS21145; ABS21145; Bcer98_0807
GeneIDi33896173
KEGGibcy:Bcer98_0807

Similar proteinsi

Entry informationi

Entry nameiGLPK_BACCN
AccessioniPrimary (citable) accession number: A7GLY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health