Reviewed, UniProtKB/Swiss-Prot A7GKJ4 (ROCA_BACCN)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	Bcer98_0289

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 H(2)O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase		PRO_1000083339
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GKJ4-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: F9A75ECE9538EF06	FASTA	515	56,146
	10 20 30 40 50 60 MVVAYKHEPF TDFSVEANKL AFEEGLKKVE SYLGQDYPLI IGGEKITTED KIVSVNPANK 70 80 90 100 110 120 EEVIGSVSKA SRELAEKAMQ VADETFQTWR KSKPEMRADI LFRAAAIVRR RKHEFSAILV 130 140 150 160 170 180 KEAGKPWNEA DADTAEAIDF MEYYGRQMLK LKDGIPVESR PIEYNRFSYI PLGVGVIISP 190 200 210 220 230 240 WNFPFAIMAG MTTAALVSGN TVLLKPASTT PVVAAKFMEV LEEAGLPAGV VNFIPGSGSE 250 260 270 280 290 300 VGDYLVDHPR TRFISFTGSR DVGIRIYERA AKVHPGQIWL KRVIAEMGGK DTIVVDKEAD 310 320 330 340 350 360 LELAAKSIVA SAFGFSGQKC SACSRAVIHE EVYDQVLNRA VELTKELTVG NPAEKGTNMG 370 380 390 400 410 420 PVNDQAAFDK VMSYVAIGKE EGKIVAGGEG DDSKGWFIQP TIVADVAEDA RLMKEEIFGP 430 440 450 460 470 480 VVAFCKAKDF DHALAIANNT EYGLTGAVIS NNRAHIEKAR EDFHVGNLYF NRGCTGAIVG 490 500 510 YQPFGGFNMS GTDSKAGGPD YLALHMQAKT TSEML

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000764 Genomic DNA. Translation: ABS20652.1.
RefSeq	YP_001373647.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5347272.
GenomeReviews	Gene locus Bcer98_0289 in contig CP000764_GR.
KEGG	bcy:Bcer98_0289.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00733. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR005932. d-1-pyrroline-5-COlate_DHase-2. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_BACCN

Accession

Primary (citable) accession number: A7GKJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)