A7GKH8 (PURL_BACCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Bcer98_0273

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 739	739	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000080547
Regions
	Nucleotide binding	111 – 122	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7GKH8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 94F709F61A545476	FASTA	739	80,334
	10 20 30 40 50 60 MSLMLEPNPT QIKEERIYAE MGLTDEEFAM IEKILGRLPN YTETGLFSVM WSEHCSYKNS 70 80 90 100 110 120 KPVLRKFPTT GERVLQGPGE GAGIVDIGDN QAVVFKMESH NHPSAIEPYQ GAATGVGGII 130 140 150 160 170 180 RDIFSMGARP VALLNSLRFG ELQSPRVKYL FEEVVAGIAG YGNCIGIPTV GGEVQFDPCY 190 200 210 220 230 240 EGNPLVNAMC VGLIHHEDIK KGQAHGAGNT VMYVGASTGR DGIHGATFAS EELSESSEAK 250 260 270 280 290 300 RPAVQVGDPF MEKLLIEACL ELIQSDALVG IQDMGAAGLT SSSAEMASKA GMGIEMYLDD 310 320 330 340 350 360 VPQRETGMTP YEMMLSESQE RMLIVVKKGR EQEVVELFEK YGLAAVAMGK VTEDKMLRLF 370 380 390 400 410 420 HKGEMVAEVP ADALAEEAPI YHKPSKEAAY FREFQEMKMK TPKVDNYKET LLALLQQPTI 430 440 450 460 470 480 ASKEWVYDQY DYQVRTSTVV TPGSDAAVVR VRGTEKALAI TTDCNSRYIY LDPETGGKIA 490 500 510 520 530 540 VAEAARNIVC SGGEPLAITD CLNFGNPEKP EIFWQIEKSV DGMSEACRTL QTPVIGGNVS 550 560 570 580 590 600 MYNERSGEAV YPTPTVGMVG LVHDLKHVTT QEFKQAGDLI YVMGETKAEF GGSELQKMMY 610 620 630 640 650 660 GKIFGQSPSI DLEVEAKRQK QLLEAIQAGL VQSAHDVAEG GLAVAIAESA IGAKGLGATV 670 680 690 700 710 720 KLAGEATAAL FAESQSRFVV TVKREQQEAF EKVVEAIQVG EVTNTNEVTI HNEENEVLLT 730 ANVDEMRKAW KGAIPCLLK

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NVH 391-98.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000764 Genomic DNA. Translation: ABS20636.1.
RefSeq	YP_001373631.1. NC_009674.1.
3D structure databases
ProteinModelPortal	A7GKH8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GKH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000039586; EBBACP00000038637; EBBACG00000039577.
GeneID	5344702.
GenomeReviews	Gene locus Bcer98_0273 in contig CP000764_GR.
KEGG	bcy:Bcer98_0273.
PATRIC	18928818. VBIBacCyt128034_0297.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
GeneTree	EBGT00050000002932.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	BCER315749:BCER98_0273-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_BACCN

Accession

Primary (citable) accession number: A7GKH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program