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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi300Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 2UniRule annotation1
Metal bindingi315Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 229ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Bcer98_0232
OrganismiBacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Taxonomic identifieri315749 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000747621 – 362D-alanine--D-alanine ligaseAdd BLAST362

Interactioni

Protein-protein interaction databases

STRINGi315749.Bcer98_0232

Structurei

3D structure databases

ProteinModelPortaliA7GKE0
SMRiA7GKE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 346ATP-graspUniRule annotationAdd BLAST206

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE
OrthoDBiPOG091H06GK

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A7GKE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKIKLGLLY GGKSAEHQVS LQTALAAIKA LNQEKFEIHP IYITEQGQWM
60 70 80 90 100
RGERIEGEVT SVQALQMSGE ANAISPVSLS TEIIPAPASK QEEAIDVIFP
110 120 130 140 150
LLHGPNGEDG TVQGLLELMN IPYVGNGVLA SAAGMDKVVM KNIFAEAGLN
160 170 180 190 200
QAKYTSFVRS VWEKNREEAY EKVEEALGYP CFVKPANLGS SVGINKCKDR
210 220 230 240 250
EELEKAFEEA FQFDRKIIVE ENIIGREVEV GVLGNDDPKC SVVGEIVPKK
260 270 280 290 300
EFYDYKSKYI DGDTALIIPA EITEEETATI QRDAIRAFQA LDGAGLTRAD
310 320 330 340 350
FFLTKDGEVY INEVNTMPGF TPFSMFPLLW QHTGLSYPEL IEELIRLAIE
360
RHKEKQKIKY TI
Length:362
Mass (Da):40,270
Last modified:September 11, 2007 - v1
Checksum:i3F49BC1B3A32569B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA Translation: ABS20598.1
RefSeqiWP_011983359.1, NC_009674.1

Genome annotation databases

EnsemblBacteriaiABS20598; ABS20598; Bcer98_0232
GeneIDi33895559
KEGGibcy:Bcer98_0232

Similar proteinsi

Entry informationi

Entry nameiDDL_BACCN
AccessioniPrimary (citable) accession number: A7GKE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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