Reviewed, UniProtKB/Swiss-Prot A7GKE0 (DDL_BACCN)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	Bcer98_0232

Organism

Bacillus cereus subsp. cytotoxis (strain NVH 391-98) [Complete proteome] [HAMAP]

Taxonomic identifier

315749 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	D-alanine--D-alanine ligase		PRO_1000074762
Regions
	Domain	141 – 346	206	ATP-grasp
	Nucleotide binding	174 – 229	56	ATP By similarity
Sites
	Metal binding	300	1	Magnesium or manganese 1 By similarity
	Metal binding	313	1	Magnesium or manganese 1 By similarity
	Metal binding	313	1	Magnesium or manganese 2 By similarity
	Metal binding	315	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GKE0-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 3F49BC1B3A32569B	FASTA	362	40,270
	10 20 30 40 50 60 MTKIKLGLLY GGKSAEHQVS LQTALAAIKA LNQEKFEIHP IYITEQGQWM RGERIEGEVT 70 80 90 100 110 120 SVQALQMSGE ANAISPVSLS TEIIPAPASK QEEAIDVIFP LLHGPNGEDG TVQGLLELMN 130 140 150 160 170 180 IPYVGNGVLA SAAGMDKVVM KNIFAEAGLN QAKYTSFVRS VWEKNREEAY EKVEEALGYP 190 200 210 220 230 240 CFVKPANLGS SVGINKCKDR EELEKAFEEA FQFDRKIIVE ENIIGREVEV GVLGNDDPKC 250 260 270 280 290 300 SVVGEIVPKK EFYDYKSKYI DGDTALIIPA EITEEETATI QRDAIRAFQA LDGAGLTRAD 310 320 330 340 350 360 FFLTKDGEVY INEVNTMPGF TPFSMFPLLW QHTGLSYPEL IEELIRLAIE RHKEKQKIKY TI

References

[1]

"Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity." Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C., Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V., Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J., Ehrlich S.D., Sorokin A. Chem. Biol. Interact. 171:236-249(2008) [PubMed: 17434157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000764 Genomic DNA. Translation: ABS20598.1.
RefSeq	YP_001373593.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5344614.
GenomeReviews	Gene locus Bcer98_0232 in contig CP000764_GR.
KEGG	bcy:Bcer98_0232.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BACCN

Accession

Primary (citable) accession number: A7GKE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	November 25, 2008
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)