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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei255ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Bcer98_0082
OrganismiBacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
Taxonomic identifieri315749 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000743161 – 485Glutamate--tRNA ligaseAdd BLAST485

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi315749.Bcer98_0082

Structurei

3D structure databases

ProteinModelPortaliA7GJZ9
SMRiA7GJZ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi252 – 256"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK09698
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7GJZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKQVRVRYA PSPTGHLHIG NARTALFNYL FARHQDGKFI IRIEDTDVKR
60 70 80 90 100
NVAGGEESQL KYLKWLGMDW DEGVDVGGKY GPYRQTERLD IYKELYEDLL
110 120 130 140 150
ERGLAYKCYM TEEELEAERE GQIARGETPR YAGNHRNLTE EQIAQFEAEG
160 170 180 190 200
RVPSIRFRVP DNREYKFNDI VKGDVTFHSN DFGDFVIVKK DGIPTYNFAV
210 220 230 240 250
AVDDHLMEIT HVLRGDDHIS NTPKQMMIYE AFGWDTPQFG HMTLIVNESR
260 270 280 290 300
KKLSKRDESI IQFIEQYEAL GYLPEAIFNF IALLGWSPVG EEEIFSKDEF
310 320 330 340 350
IKMFDAARLS KSPALFDSQK LKWMNNQYMK KQDLDTVVAL SLPHLVKAGR
360 370 380 390 400
VSEDLSEQEA AWVRDVIALY HEQMSYGAEI VELSEMFFKD HVDFEEEGKE
410 420 430 440 450
VLKGEQVPEV LRAFAVELEA LEEVEPATIK KAIKAVQKET GHKGKNLFMP
460 470 480
IRVATTGQTH GPELPNAIAL LGKEKVLKRI QKVIG
Length:485
Mass (Da):55,661
Last modified:September 11, 2007 - v1
Checksum:iCB16F2A5299F1549
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000764 Genomic DNA Translation: ABS20457.1
RefSeqiWP_011983226.1, NC_009674.1

Genome annotation databases

EnsemblBacteriaiABS20457; ABS20457; Bcer98_0082
GeneIDi33895403
KEGGibcy:Bcer98_0082

Similar proteinsi

Entry informationi

Entry nameiSYE_BACCN
AccessioniPrimary (citable) accession number: A7GJZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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