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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT441772:GJIE-3689-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:CLI_3732
OrganismiClostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Taxonomic identifieri441772 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Formate--tetrahydrofolate ligasePRO_0000318568Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA7GJB8.
SMRiA7GJB8. Positions 5-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7GJB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKSDIEIAQ ESKMKNIKNI AEKIGLTEED IDLYGKYKCK ISLDVLESNK
60 70 80 90 100
DKKDGKLILV TAINPTPAGE GKSTVTVGLG QALWKKNKKA VIALREPSLG
110 120 130 140 150
PVFGIKGGAA GGGYSQVVPM EDINLHFTGD MHAITSANNL LAAAIDNHIH
160 170 180 190 200
QGNILKIDQR RILFKRVMDM NDRALRNVIV ALGGKINGFP REDGFMITVA
210 220 230 240 250
SEIMAILCLA EDLMDLKNKM GEILVAYSTE GKPIYCKDLE VQGAMALLMK
260 270 280 290 300
DAIKPNLVQT LENTPAIIHG GPFANIAHGC NSILGTKMAL KLGDYVITEA
310 320 330 340 350
GFGADLGAEK FFDIKCRKAN LKPNCVVIVA TVRALKYNGG IPKENLKEQN
360 370 380 390 400
MEALSKGIKN LGKHIENVNK FGVPAVVAIN KFISDTEEEI EFIKKYCKEL
410 420 430 440 450
GAEVSIAEVW EKGGNGGLEL ADKVLDTIEN KESKFNPIYE ETLNIKQKIE
460 470 480 490 500
TIAQEIYGAE GVDYSKEAEK QISEIEKLDL DKKPVCMAKT QYSLSDDAKL
510 520 530 540 550
LGRPCGFRIN VKEVRISNGA GFIVVLTGNV MTMPGLPKKP AANNMDVLSD

GNIVGLF
Length:557
Mass (Da):60,701
Last modified:September 11, 2007 - v1
Checksum:i9A40CF97CAA27596
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000728 Genomic DNA. Translation: ABS41963.1.
RefSeqiWP_003399652.1. NC_009699.1.

Genome annotation databases

EnsemblBacteriaiABS41963; ABS41963; CLI_3732.
KEGGicbf:CLI_3732.
PATRICi19430371. VBICloBot15611_3583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000728 Genomic DNA. Translation: ABS41963.1.
RefSeqiWP_003399652.1. NC_009699.1.

3D structure databases

ProteinModelPortaliA7GJB8.
SMRiA7GJB8. Positions 5-555.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS41963; ABS41963; CLI_3732.
KEGGicbf:CLI_3732.
PATRICi19430371. VBICloBot15611_3583.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCBOT441772:GJIE-3689-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Langeland / NCTC 10281 / Type F.

Entry informationi

Entry nameiFTHS_CLOBL
AccessioniPrimary (citable) accession number: A7GJB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.