A7GGI0 (OTC_CLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	CLI_2656

Organism

Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]

Taxonomic identifier

441772 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_1000065089
Regions
	Region	56 – 60	5	Carbamoyl phosphate binding By similarity
	Region	272 – 275	4	Ornithine binding By similarity
Sites
	Binding site	107	1	Carbamoyl phosphate By similarity
	Binding site	134	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	147	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GGI0 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 0F4E4F5E8F8D217E	FASTA	333	37,252
	10 20 30 40 50 60 MFNLKNRNFL TLMDFTPKEI NYFLDLARDL KRAKYTGTEV QRMKGKNIAL IFEKASTRTR 70 80 90 100 110 120 CAFEVGAKDQ GAHVTYLGPT GSHIGKKESA ADTARVLGRM YDGIEYRGFG QEIVETLAEY 130 140 150 160 170 180 AGVPVWNGLT DEDHPTQILA DFLTIREHFN KPLSEIKFAY VGDGANNMAN ALMIGAVKMG 190 200 210 220 230 240 MDFRIVSPKE IPTDATLVAK CKEIAAETGA KVTITDNIEE GVKGCDVLYT DVWVSMGEPD 250 260 270 280 290 300 SVWESKIKLL TPYRVDMNMI KMTGNPDAKF MHCLPAFHDE ETAVGKEIKE KYGLSEMEVS 310 320 330 HELFESKYSI VFDEAENRMH TIKAVMVATL GDQ

References

[1]	Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Langeland / NCTC 10281 / Type F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000728 Genomic DNA. Translation: ABS42372.1.
RefSeq	YP_001391890.1. NC_009699.1.
3D structure databases
ProteinModelPortal	A7GGI0.
SMR	A7GGI0. Positions 2-332.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GGI0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5401857.
GenomeReviews	Gene locus CLI_2656 in contig CP000728_GR.
KEGG	cbf:CLI_2656.
PATRIC	19428253. VBICloBot15611_2568.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0078.
HOGENOM	HBG579429.
OMA	MTPYQIN.
ProtClustDB	PRK04284.
Enzyme and pathway databases
BioCyc	CBOT441772:CLI_2656-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_CLOBL

Accession

Primary (citable) accession number: A7GGI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program