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A7GFJ9

- BIOB_CLOBL

UniProt

A7GFJ9 - BIOB_CLOBL

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Protein
Biotin synthase
Gene
bioB, CLI_2309
Organism
Clostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi268 – 2681Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT441772:GJIE-2281-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:CLI_2309
OrganismiClostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Taxonomic identifieri441772 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002410: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Biotin synthaseUniRule annotation
PRO_0000381315Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi441772.CLI_2309.

Structurei

3D structure databases

ProteinModelPortaliA7GFJ9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRDFHGMA.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A7GFJ9-1 [UniParc]FASTAAdd to Basket

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MSNIIKYKKK ILNGDLLTKE EVEEILEEDI TDLAATANEI RESLCGNKFD    50
LCTIINGKSG RCQENCKYCA QSAHFDTDII EYNILNSDRI INSAISNYNK 100
GVHRFSVVTS GRALNNNEVD TLCKTYSKLK ETCSIRLCAS HGLLKYEDLK 150
RLKDSGVTRY HNNLETSRKF FTKICTTHKY DDKIETIKNA KKAGLEICSG 200
GIIGLGETME DRIDMAFTLR ELSVESVPVN ILNPIKGTPL ENQEILSYEE 250
IIKTLALFRF ILPTVQIRLA GGRTIISDNG KKALESGVNG AISGDMLTTL 300
GIETSEDIKM IKNLGFEV 318
Length:318
Mass (Da):35,492
Last modified:September 11, 2007 - v1
Checksum:iE4F47E6AF942F48D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000728 Genomic DNA. Translation: ABS42726.1.
RefSeqiWP_012100230.1. NC_009699.1.
YP_001391559.1. NC_009699.1.

Genome annotation databases

EnsemblBacteriaiABS42726; ABS42726; CLI_2309.
GeneIDi5402366.
KEGGicbf:CLI_2309.
PATRICi19427557. VBICloBot15611_2220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000728 Genomic DNA. Translation: ABS42726.1 .
RefSeqi WP_012100230.1. NC_009699.1.
YP_001391559.1. NC_009699.1.

3D structure databases

ProteinModelPortali A7GFJ9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 441772.CLI_2309.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS42726 ; ABS42726 ; CLI_2309 .
GeneIDi 5402366.
KEGGi cbf:CLI_2309.
PATRICi 19427557. VBICloBot15611_2220.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RDFHGMA.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CBOT441772:GJIE-2281-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Langeland / NCTC 10281 / Type F.

Entry informationi

Entry nameiBIOB_CLOBL
AccessioniPrimary (citable) accession number: A7GFJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 11, 2007
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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