A7GFJ8 (BIOD_CLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	CLI_2308

Organism

Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]

Taxonomic identifier

441772 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000019558
Regions
	Nucleotide binding	116 – 119	4	ATP By similarity
	Nucleotide binding	179 – 180	2	ATP By similarity
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	55	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	55	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GFJ8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: E3412F5ABC8946A7	FASTA	227	25,428
	10 20 30 40 50 60 MARGIFITAT GTDIGKTYVT ALIIKRLRET NINCGYYKAA LSGAERRDGK LIAGDANYVY 70 80 90 100 110 120 NIANIKGDPN DAVSYIFQQA VSPHLAAKLN NVEISMERIK KDFYSIKNKY DYITVEGSGG 130 140 150 160 170 180 IVCPISTGKE KIMLDNIIKI FKLPAIVVAD AGLGTINSTI LTLQYMKEKN ISVKMILLNN 190 200 210 220 YNHEDIIHIE NKRYLSDNLL IPVYTCNKNA NKLEIPVEKL IEIYEEI

References

[1]	Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Langeland / NCTC 10281 / Type F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000728 Genomic DNA. Translation: ABS42436.1.
RefSeq	YP_001391558.1. NC_009699.1.
3D structure databases
ProteinModelPortal	A7GFJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GFJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5402203.
GenomeReviews	Gene locus CLI_2308 in contig CP000728_GR.
KEGG	cbf:CLI_2308.
PATRIC	19427555. VBICloBot15611_2219.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	LNNYNHE.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	CBOT441772:CLI_2308-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOBL

Accession

Primary (citable) accession number: A7GFJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program