A7GDI9 (A7GDI9_CLOBL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555 EMBL ABS40806.1
Ordered Locus Names:	CLI_1585

Organism

Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]

Taxonomic identifier

441772 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555 SAAS SAAS004618
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555 SAAS SAAS004618
Subcellular location	Cytoplasm By similarity HAMAP MF_00555 SAAS SAAS004618.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555 SAAS SAAS004618
Cellular component	Cytoplasm HAMAP MF_00555 SAAS SAAS004618
Ligand	ATP-binding HAMAP MF_00555 SAAS SAAS004618 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 SAAS SAAS004618 EMBL ABS40806.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A7GDI9 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 9B43EAFD67D41A4F	FASTA	340	39,508
	10 20 30 40 50 60 MIKFKDRLMI PEGYESTLGI RETEVAIKKV KDFFERTLAE KLNITRVSAP LFVRKNTGMN 70 80 90 100 110 120 DNLNGVERPV AFDMKDLQDE MIEIVHSLAK WKRMALHRYD FKVGEGLYTD MNAIRRDEDL 130 140 150 160 170 180 DNLHSIYVDQ WDWEKVIKKE DRNKETLKAI VNKIYSAFKE TERYICSQYE GFNEILPEEI 190 200 210 220 230 240 CFITSQELQD TYPNLDSKER EDAITKEKGA VFLMEIGGVL ASGEKHDGRA PDYDDWTLNG 250 260 270 280 290 300 DILFWNPVLE RAFELSSMGI RVDEEALEKQ LEITGCEDRK NLEFHRLLLE GKLPYTVGGG 310 320 330 340 IGQSRICMYF LRKAHIGEVQ ASIWPDLMIE DCEKAKIKLL

References

[1]	Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000728 Genomic DNA. Translation: ABS40806.1.
RefSeq	YP_001390849.1. NC_009699.1.
3D structure databases
ProteinModelPortal	A7GDI9.
SMR	A7GDI9. Positions 21-334.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GDI9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5403771.
GenomeReviews	Gene locus CLI_1585 in contig CP000728_GR.
KEGG	cbf:CLI_1585.
PATRIC	19426123. VBICloBot15611_1503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A7GDI9_CLOBL

Accession

Primary (citable) accession number: A7GDI9

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)