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Protein

Ribosomal RNA small subunit methyltransferase H

Gene

rsmH

Organism
Clostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531S-adenosyl-L-methionineUniRule annotation
Binding sitei79 – 791S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei100 – 1001S-adenosyl-L-methionineUniRule annotation
Binding sitei107 – 1071S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT441772:GJIE-1515-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase HUniRule annotation (EC:2.1.1.199UniRule annotation)
Alternative name(s):
16S rRNA m(4)C1402 methyltransferaseUniRule annotation
rRNA (cytosine-N(4)-)-methyltransferase RsmHUniRule annotation
Gene namesi
Name:rsmHUniRule annotation
Synonyms:mraW
Ordered Locus Names:CLI_1538
OrganismiClostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Taxonomic identifieri441772 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000002410 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Ribosomal RNA small subunit methyltransferase HPRO_0000386816Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA7GDE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 353S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000049777.
KOiK03438.
OMAiHVPVMLQ.
OrthoDBiEOG6X9MQ1.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.

Sequencei

Sequence statusi: Complete.

A7GDE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFKHISVLL EETIDSLNIK EDGVYVDCTL GGGGHSKEIL KKLSHKGKLI
60 70 80 90 100
GIDQDTSAIK AAKEKLKDYE NIIYVHNNFY NIDSILEELD IDKVDGIIMD
110 120 130 140 150
LGVSSYQLDE ASRGFSYMKD APLDMRMNRE ENFSAYNVVN SYEEEELFKI
160 170 180 190 200
LKNYGEEKFS RKIARFIVEK RTENPIETTG ELVEIIRKAI PAKFQREGHP
210 220 230 240 250
AKRTFQAIRI EVNKELQILN KAIEDSVNRL NKDGRLSIIT FHSLEDRIVK
260 270 280 290 300
VKFKELEKPC TCPPSFPICV CGKEPQIKII TKKPIEPSKE EKEINSRSRS

AKLRVCRKI
Length:309
Mass (Da):35,527
Last modified:September 11, 2007 - v1
Checksum:i5F33D587B80D9F87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000728 Genomic DNA. Translation: ABS40085.1.
RefSeqiWP_011988264.1. NC_009699.1.

Genome annotation databases

EnsemblBacteriaiABS40085; ABS40085; CLI_1538.
KEGGicbf:CLI_1538.
PATRICi19426029. VBICloBot15611_1456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000728 Genomic DNA. Translation: ABS40085.1.
RefSeqiWP_011988264.1. NC_009699.1.

3D structure databases

ProteinModelPortaliA7GDE2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS40085; ABS40085; CLI_1538.
KEGGicbf:CLI_1538.
PATRICi19426029. VBICloBot15611_1456.

Phylogenomic databases

HOGENOMiHOG000049777.
KOiK03438.
OMAiHVPVMLQ.
OrthoDBiEOG6X9MQ1.

Enzyme and pathway databases

BioCyciCBOT441772:GJIE-1515-MONOMER.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Langeland / NCTC 10281 / Type F.

Entry informationi

Entry nameiRSMH_CLOBL
AccessioniPrimary (citable) accession number: A7GDE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: September 11, 2007
Last modified: November 11, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.