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A7GCD9

- SYE_CLOBL

UniProt

A7GCD9 - SYE_CLOBL

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium botulinum (strain Langeland / NCTC 10281 / Type F)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081ZincUniRule annotation
    Metal bindingi110 – 1101ZincUniRule annotation
    Metal bindingi135 – 1351ZincUniRule annotation
    Metal bindingi137 – 1371ZincUniRule annotation
    Binding sitei255 – 2551ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCBOT441772:GJIE-1159-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:CLI_1183
    OrganismiClostridium botulinum (strain Langeland / NCTC 10281 / Type F)
    Taxonomic identifieri441772 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000002410: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Glutamate--tRNA ligasePRO_0000330963Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi441772.CLI_1183.

    Structurei

    3D structure databases

    ProteinModelPortaliA7GCD9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi11 – 2111"HIGH" regionAdd
    BLAST
    Motifi252 – 2565"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiWINGYYL.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7GCD9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNKVRTRFA PSPTGYMHVG NLRTALYAYL IAKHDNGDFI LRIEDTDQER    50
    LVEGALDVIY NTLKITGLSH DEGPDIGGPV GPYVQSERRN IYIEYAEKLI 100
    EKGEAYYCFC SKERLDMLRA NSEALKRPFR YDKHCIDLSK EEIDKKIAEG 150
    VPYVIRQKNP TTGSTSFHDE IYGDISVDNS ELDDMILIKS DGLPTYNFAN 200
    VVDDHLMGIT HVVRGSEYLS SSPKYNRLYE AFGWDVPIYV HCPPIMKDEH 250
    HKLSKRNGDA SFEDLMAKGY LKEAILNYIA LLGWNPGGEK EVFSMEELIE 300
    AFNYRNINKA PAVFDTKKLK WMNGEYIRAL SLDKFHEMAL PYYEEALTRD 350
    LDTKKISELL HTRVEVLNEI PEQLDFFNNL LEYSPEMYIH KKMKTTYENS 400
    LKSLEEVLPK LEALENWTFE NIKEVCMNLV KELEVKNGVV LWPIRTAVSG 450
    KQFTPGGAFE IADILGKEET LERIKIGIDK LKALQ 485
    Length:485
    Mass (Da):55,743
    Last modified:September 11, 2007 - v1
    Checksum:i4D4264727F64BBB2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000728 Genomic DNA. Translation: ABS39708.1.
    RefSeqiYP_001390449.1. NC_009699.1.

    Genome annotation databases

    EnsemblBacteriaiABS39708; ABS39708; CLI_1183.
    GeneIDi5405249.
    KEGGicbf:CLI_1183.
    PATRICi19425325. VBICloBot15611_1104.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000728 Genomic DNA. Translation: ABS39708.1 .
    RefSeqi YP_001390449.1. NC_009699.1.

    3D structure databases

    ProteinModelPortali A7GCD9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 441772.CLI_1183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS39708 ; ABS39708 ; CLI_1183 .
    GeneIDi 5405249.
    KEGGi cbf:CLI_1183.
    PATRICi 19425325. VBICloBot15611_1104.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi WINGYYL.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci CBOT441772:GJIE-1159-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Langeland / NCTC 10281 / Type F.

    Entry informationi

    Entry nameiSYE_CLOBL
    AccessioniPrimary (citable) accession number: A7GCD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3