A7GB83 (GCST_CLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	CLI_0765

Organism

Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]

Taxonomic identifier

441772 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Aminomethyltransferase HAMAP MF_00259		PRO_1000047658

Sequences

	Sequence		Length	Mass (Da)
	A7GB83 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: BE439D8D24749CAB	FASTA	370	41,529
	10 20 30 40 50 60 MEDLKVTPLR GVYEEYGGKI VDFAGYELPT QFKGFLHEHH TVREKAGLFD VSHMGEAMVT 70 80 90 100 110 120 GKDAGKFIQY LMTNDINVLK DNEVLYTFMC NEDGGVIDDL LVYKFAGDEF FLVINASNKD 130 140 150 160 170 180 KDVKWIMGHK GDFDVEIVDV SDSIAQLAFQ GPLAEEILQK IVDVDLQEIK FFKLKRDVLV 190 200 210 220 230 240 DGKKCLVSRT GYTGEDGFEI YCKPEDAKGL WHAILNAGKE EGAQPIGLGA RDTLRFEASL 250 260 270 280 290 300 LLYGNEMDET ITPLEVGMGF FVKLKVEEDF IGKDALIKQK AEGITRKLVG FELLDKGIPR 310 320 330 340 350 360 HGYEVIKDGK VIGHVTTGYK SPTLNKAIGL ALVEEQYSKI GTEFNIKVRK KELKAVAIDK 370 RFYTKKTKTK

References

[1]	Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Langeland / NCTC 10281 / Type F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000728 Genomic DNA. Translation: ABS40921.1.
RefSeq	YP_001390043.1. NC_009699.1.
3D structure databases
ProteinModelPortal	A7GB83.
SMR	A7GB83. Positions 4-362.
ModBase	Search...
Protein-protein interaction databases
STRING	A7GB83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5404760.
GenomeReviews	Gene locus CLI_0765 in contig CP000728_GR.
KEGG	cbf:CLI_0765.
PATRIC	19424493. VBICloBot15611_0688.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
HOGENOM	HBG299834.
OMA	VINASNK.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	CBOT441772:CLI_0765-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_CLOBL

Accession

Primary (citable) accession number: A7GB83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program