A7GAL1 (THIM1_CLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase 1
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 1
Short name=TH kinase 1
Short name=Thz kinase 1

Gene names

Name:	thiM1
Ordered Locus Names:	CLI_0535

Organism

Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]

Taxonomic identifier

441772 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum F

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Hydroxyethylthiazole kinase 1 HAMAP-Rule MF_00228		PRO_0000336549
Sites
	Binding site	42	1	Substrate; via amide nitrogen By similarity
	Binding site	118	1	ATP By similarity
	Binding site	164	1	ATP By similarity
	Binding site	191	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7GAL1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 8F3C24E8D5D034BE	FASTA	263	28,464
	10 20 30 40 50 60 MKNKNVIQKM REKIPLIHCI TNYVTINDCA NILLSFGASP AMCEAYDEVY DFVSISSALY 70 80 90 100 110 120 INLGTLTKEQ ETAAVLASIS AKNHNVPVVI DPVGCPAIKR KVEVINRIAE VGRIDIIKGN 130 140 150 160 170 180 IGEIKFLAGM DSETRGVDSL DNGENALNAC TQLAKKYNCI VAATGQKDFV SDGKRGSVIK 190 200 210 220 230 240 NGTEMLTKVT GAGCMLGALC AATCANFEDK LVSTTAAILS MNIAGEKAYE KAQLPGSFRI 250 260 ALIDNIYMIS DEEIWERGNV EWK

References

[1]	Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Langeland / NCTC 10281 / Type F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000728 Genomic DNA. Translation: ABS41348.1.
RefSeq	YP_001389821.1. NC_009699.1.
3D structure databases
ProteinModelPortal	A7GAL1.
ModBase	Search...
Protein-protein interaction databases
STRING	441772.CLI_0535.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS41348; ABS41348; CLI_0535.
GeneID	5405245.
KEGG	cbf:CLI_0535.
PATRIC	19424032. VBICloBot15611_0459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HOG000114352.
KO	K00878.
OMA	ENGTEML.
ProtClustDB	CLSK950618.
Enzyme and pathway databases
BioCyc	CBOT441772:GJIE-514-MONOMER.
UniPathway	UPA00060; UER00139.
Family and domain databases
HAMAP	MF_00228. Thz_kinase.
InterPro	IPR000417. Hyethyz_kinase. IPR026012. Hyethyz_kinase/Th_synth. [Graphical view]
PANTHER	PTHR20857:SF14. PTHR20857:SF14. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM1_CLOBL

Accession

Primary (citable) accession number: A7GAL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program