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A7G9T9 (SYL_CLOBL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CLI_0249
OrganismClostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]
Taxonomic identifier441772 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009327

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif572 – 5765"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5751ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7G9T9 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: A6D241BCDEBEEC0D

FASTA81393,072
        10         20         30         40         50         60 
MGNYSTKIDE KWQKKWEENS LYKFNNDNLD KKLYVLEMFS YPSGSKLHAG HWFNYGPVDS 

        70         80         90        100        110        120 
WARFKRMQGY NVFQPMGFDA FGLPAENYAI KTGIHPKDST FKNIETMETQ LKAMGAMFNW 

       130        140        150        160        170        180 
ENEVITCSPD YYKWTQWLFL KLYEKGLAYK KKAPVNWCPS CNTVLANEQV LDGKCERCDS 

       190        200        210        220        230        240 
NVDKKNLEQW FLKITDYADE LLEKLDELDW PEKTKAMQKH WIGKSVGAEV TFNVADSDLS 

       250        260        270        280        290        300 
FNVFTTRVDT LFGVTYVVLA PENDLVDKLT TPENKAEVES YKTQAKNQSD IERQSITREK 

       310        320        330        340        350        360 
TGVFSGSYAI NPINGKKVPI WIGDYVLNTY GTGCVMAVPA HDERDFAFAT KYNLPIERVI 

       370        380        390        400        410        420 
EGGDSLPYTE YGKMVNSGEF DGLFGNKAKE AVISKLESMN LGRKKINYRL RDWLVSRQRY 

       430        440        450        460        470        480 
WGAPIPIIYC EKCGTVEVPI EQLPVELPYN VEFSPDGKSP LGKCDDFINT TCPKCGGPAK 

       490        500        510        520        530        540 
READTLDTFV CSSWYYLRYP DNNNEKDAFN PELINKMLPV DKYVGGPEHA CMHLLYARFI 

       550        560        570        580        590        600 
TKALRDMGYL NFDEPFLSLT HQGLILGPDG LKMSKSKGNT ISPDDYIKEF GADVFRMYLM 

       610        620        630        640        650        660 
FGFDYTEGGA WSDDAIKSIG KFVDRVERIL ENAREEIKNS KDNKSTMDKD EKELNYVRHH 

       670        680        690        700        710        720 
SIKSITEDID KMQFNTSIAR LMEFTNALSK YLGSDTLRNA SFLRESIIDF ITLLAPFAPH 

       730        740        750        760        770        780 
FAEEQWELIG INSSIFNEKW PEFDPKALIK DEVEIAVQVN GKIRAKINIY TSSSEDEIKE 

       790        800        810 
SALNNDDIKN SIGDKEIKKV IVIKNRLVNI VAK 

« Hide

References

[1]Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Langeland / NCTC 10281 / Type F.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000728 Genomic DNA. Translation: ABS39371.1.
RefSeqYP_001389549.1. NC_009699.1.

3D structure databases

ProteinModelPortalA7G9T9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING441772.CLI_0249.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS39371; ABS39371; CLI_0249.
GeneID5403523.
KEGGcbf:CLI_0249.
PATRIC19423456. VBICloBot15611_0178.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCBOT441772:GJIE-229-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CLOBL
AccessionPrimary (citable) accession number: A7G9T9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries