A7G9N9 (KITH_CLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	CLI_0191

Organism

Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) [Complete proteome] [HAMAP]

Taxonomic identifier

441772 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum F

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Thymidine kinase HAMAP-Rule MF_00124		PRO_1000018154
Regions
	Nucleotide binding	15 – 22	8	ATP By similarity
	Nucleotide binding	88 – 91	4	ATP By similarity
Sites
	Active site	89	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	148	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7G9N9 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 524B3EA8664BD59E	FASTA	191	21,545
	10 20 30 40 50 60 MYGPKDHGWI EVVAGPMYSG KTEELIRRIR RAEIAKQKVQ VFKPEIDNRY SKQDVVSHAG 70 80 90 100 110 120 DKIQSVPVKS SKEILEKLLD DTDVIGIDEA QFFDDFLVEI VSKIANNNRR VICAGLDMDF 130 140 150 160 170 180 KGEPFGPMPK LMAIAEFVDK IQAVCMVCNN PATRTQRLIN GKPAKKSDPV VLIGAQESYE 190 ARCRKCHRVP R

References

[1]	Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Langeland / NCTC 10281 / Type F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000728 Genomic DNA. Translation: ABS41898.1.
RefSeq	YP_001389499.1. NC_009699.1.
3D structure databases
ProteinModelPortal	A7G9N9.
SMR	A7G9N9. Positions 2-191.
ModBase	Search...
Protein-protein interaction databases
STRING	441772.CLI_0191.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS41898; ABS41898; CLI_0191.
GeneID	5403454.
KEGG	cbf:CLI_0191.
PATRIC	19423346. VBICloBot15611_0128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076390.
KO	K00857.
OMA	KVHAICV.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	CBOT441772:GJIE-172-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_CLOBL

Accession

Primary (citable) accession number: A7G9N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program