A7FWM4 (OTC_CLOB1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	CLB_2534

Organism

Clostridium botulinum (strain ATCC 19397 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441770 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_1000065087
Regions
	Region	56 – 60	5	Carbamoyl phosphate binding By similarity
	Region	272 – 275	4	Ornithine binding By similarity
Sites
	Binding site	107	1	Carbamoyl phosphate By similarity
	Binding site	134	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	147	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7FWM4 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 3D8C229AC983B362	FASTA	333	37,231
	10 20 30 40 50 60 MFNLKNRNFL TLMDFTPKEI NYFLDLARDL KRAKYTGTEV QRLKGKNIAL IFEKASTRTR 70 80 90 100 110 120 CAFEVGAKDQ GAHVTYLGPT GSHIGKKESA ADTARVLGRM YDGIEYRGFG QEIVETLAEY 130 140 150 160 170 180 AGVPVWNGLT DEDHPTQILA DFLTIREHFN KPLNEIKFAY VGDGANNMAN ALMIGAVKMG 190 200 210 220 230 240 MDFRIVSPKE IPTDAALVAK CKEIAAETGA KVTITDNIEE GVKGCDVLYT DVWVSMGEPD 250 260 270 280 290 300 SVWESKIKLL TPYRVDMNMI KMTGNPDAKF MHCLPAFHDE ETAVGKEIKE KYGLSEMEVS 310 320 330 HELFESKYSI VFDEAENRMH TIKAVMVATL GDQ

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19397 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000726 Genomic DNA. Translation: ABS33574.1.
RefSeq	YP_001384835.1. NC_009697.1.
3D structure databases
ProteinModelPortal	A7FWM4.
SMR	A7FWM4. Positions 2-332.
ModBase	Search...
Protein-protein interaction databases
STRING	A7FWM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5396752.
GenomeReviews	Gene locus CLB_2534 in contig CP000726_GR.
KEGG	cba:CLB_2534.
PATRIC	19359618. VBICloBot110701_2444.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0078.
HOGENOM	HBG579429.
OMA	MTPYQIN.
ProtClustDB	PRK04284.
Enzyme and pathway databases
BioCyc	CBOT441770:CLB_2534-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_CLOB1

Accession

Primary (citable) accession number: A7FWM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program